{"product_id":"recombinant-human-prolyl-endopeptidase-fap-fap-partial-active-bhp10516665","title":"Recombinant Human Prolyl endopeptidase FAP (FAP), partial (Active)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Human Prolyl endopeptidase FAP (FAP), partial (Active) is a recombinant protein preparation derived from Homo sapiens (Human). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 26-760aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Mammalian cell (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e N-terminal 10xHis-tagged; Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 88.7 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eCell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala\/Ser-Gly-Pro-Ser\/Asn\/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure FAP enzymatic activity with defined substrates\/cofactors (in vitro assay).\u003c\/li\u003e\n\u003cli\u003eEvaluate inhibitor\/activator effects on FAP activity across a concentration series.\u003c\/li\u003e\n\u003cli\u003eQuantify FAP using calibration standards in plate-based assays (assay development).\u003c\/li\u003e\n\u003cli\u003eProfile binding interactions of FAP by SPR\/BLI (kinetic characterization).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (Q12884) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/Q12884 - NCBI Gene search (FAP) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FAP - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FAP - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059325034861,"sku":"CSB-MP008424HU-1MG","price":3168.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059494248813,"sku":"CSB-MP008424HU-100UG","price":448.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059494281581,"sku":"CSB-MP008424HU-20UG","price":256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-MP008424HU-SDS.jpg?v=1772280451","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-prolyl-endopeptidase-fap-fap-partial-active-bhp10516665","provider":"BioHippo","version":"1.0","type":"link"}