{"product_id":"recombinant-human-protein-kinase-a-regulatory-subunit-1-alpha-bhp11001684","title":"Recombinant Human Protein Kinase A regulatory subunit-1 alpha","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Human Protein Kinase A regulatory subunit-1 alpha\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eThe Regulatory\u003cbr\u003e(R) subunit of Protein Kinase A (PKA) inhibits its kinase activity by shielding the Catalytic\u003cbr\u003e(C) subunit from physiological substrates. This inhibition is reversed in response to extra-cellular signals that increase cAMP levels in the cytoplasm. Upon cAMP binding to R, C is allosterically released from R, activating a spectrum of downstream signaling cascades. Crystallographic data indicated that a series of distinct conformational changes within CBD-A must occur to relay the cAMP signal from the cAMP binding site to the R:C interaction interface. One critical cAMP relay site within the CBD-A of R has been identified as Asp170 because the D170A mutation selectively reduces the negative cooperativity between the cAMP- and C-recognition sites (i.e. the KD for the R:C complex in the presence of cAMP is reduced by more than 12-fold), without significantly compromising the high affinity of R for both binding partners.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eInhibitory Activity:\u003c\/strong\u003e The cAMP-dependent protein kinase, regulatory subunit RIa reversibly inhibits the catalytic subunit Ca of the cAMP-dependent protein kinase PKA. The inhibition of the catalytic subunit can be reversed by the addition of the second messenger cAMP.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSequences\u003c\/h3\u003e\n\u003cdetails\u003e\u003csummary\u003eAntigen Amino Acid Sequence\u003c\/summary\u003e\u003cpre style=\"white-space:pre-wrap;\"\u003eMESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL REYFERLEKE EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI SAEVYTEEDA ASYVRKVIPK DYKTMAALAK AIEKNVLFSH LDDNERSDIF DAMFSVSFIA GETVIQQGDE GDNFYVIDQG ETDVYVNNEW ATSVGEGGSF GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL RKRKMYEEFL SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGSA AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL DRPRFERVLG PCSDILKRNI QQYNSFVSLS V\u003c\/pre\u003e\u003c\/details\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"5 ug","offer_id":53038068334957,"sku":"pka-202-5UG","price":310.0,"currency_code":"USD","in_stock":true},{"title":"25 ug","offer_id":53038319796589,"sku":"pka-202-25UG","price":890.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53038319829357,"sku":"pka-202-50UG","price":1530.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-pkar-i-alpha-human-PKA-202.png?v=1782158166","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-protein-kinase-a-regulatory-subunit-1-alpha-bhp11001684","provider":"BioHippo","version":"1.0","type":"link"}