{"product_id":"recombinant-human-psca-bhp10800859","title":"Recombinant Human PSCA","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003ePSCA\u003c\/strong\u003e is provided as a recombinant protein reagent for research use only (RUO), offering a defined input for assay development and mechanistic studies.\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003ePSCA\u003c\/strong\u003e is studied in RUO research to understand \u003cstrong\u003eself-renewal and lineage commitment programs in stem\/progenitor models; oncogenic signaling, proliferation control, and tumor–microenvironment biology (RUO)\u003c\/strong\u003e and related molecular pathways. recombinant proteins are used as defined reagents to support mechanistic experiments, interaction mapping, and assay development in controlled settings.\u003c\/p\u003e\u003cp\u003eMechanistically, researchers often analyze how PSCA participates in pathway networks through molecular interactions, localization, and regulated activity. Depending on the target class, this can involve receptor-mediated signaling, enzymatic catalysis, complex assembly, or structural organization that shapes downstream cellular phenotypes.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch relevance:\u003c\/strong\u003e RUO studies frequently connect PSCA to perturbations such as immune stimulation, stress signaling, differentiation cues, metabolic remodeling, or engineered genetic modulation—then interpret downstream readouts using complementary pathway markers.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eMolecular features matter in RUO experiments:\u003c\/strong\u003e domain boundaries, oligomerization state, and PTM sensitivity can influence binding behavior, stability, and functional readouts in vitro.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConstruct \/ expression region:\u003c\/strong\u003e aa 12-86\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApprox. molecular weight:\u003c\/strong\u003e 28.9 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 90% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg\/ml by gently pipetting 2-3 times, don't vortex.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePTM considerations:\u003c\/strong\u003e Post-translational modifications (PTMs) can influence stability, binding, and activity for many proteins. Whether PTMs are present depends on expression system and protein class. Prokaryotic expression typically yields non-glycosylated protein; consider whether eukaryotic PTMs are required for your assay context.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e This protein is produced in a \u003cstrong\u003eprokaryotic (E. coli)\u003c\/strong\u003e system, which typically yields a defined, non-glycosylated form. This can be advantageous for mechanistic studies, binding assays, and antigen\/standard use where mammalian PTMs are not required.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePurification transparency (research credibility):\u003c\/strong\u003e In research-grade recombinant protein production, constructs are commonly purified via affinity and polishing steps (e.g., chromatography) to reduce contaminants and improve batch-to-batch consistency. When present, affinity tags (e.g., His\/GST\/Fc) can simplify purification; tag presence or removal can influence certain binding or structural assays.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eForm and handling context:\u003c\/strong\u003e Lyophilized proteins are frequently used in RUO labs to support stability during storage and shipment, while formulation components and reconstitution conditions can impact solubility and aggregation—important considerations when comparing studies across publications.\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e In experimental systems, changes involving \u003cstrong\u003ePSCA\u003c\/strong\u003e may reflect shifts in upstream regulation, protein stability, or interaction networks. published studies commonly emphasize combining multiple readouts to interpret mechanism rather than relying on a single measurement.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eUsing recombinant protein as a defined reagent:\u003c\/strong\u003e recombinant PSCA is commonly used as a quantitative input for assay calibration, antibody\/ligand binding studies, pathway reconstitution, and controlled perturbation experiments. Researchers often consider isoforms, fragments, or construct boundaries when comparing results across studies.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"50 ug","offer_id":53013956854125,"sku":"P1480-50UG","price":374.4,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53013956886893,"sku":"P1480-200UG","price":748.8,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53013956919661,"sku":"P1480-1MG","price":2246.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/E8_9B_8B_E7_99_BD_5ce32743-f139-47bb-8367-ba6f96a58a05.jpg?v=1770539545","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-psca-bhp10800859","provider":"BioHippo","version":"1.0","type":"link"}