{"product_id":"recombinant-human-receptor-tyrosine-protein-kinase-erbb-2-erbb2-partial-bhp10507183","title":"Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eERBB2\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 153-598aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 153-598aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Metastatic lymph node gene 19 protein ;MLN 19Proto-oncogene NeuProto-oncogene c-ErbB-2Tyrosine kinase-type cell surface receptor HER2p185erbB2;; CD340. Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell mbrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell mbrane. In turn, mbrane-bound APC allows the localization of MACF1 to the cell mbrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2\/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving ERBB2\u003c\/li\u003e\n\u003cli\u003eBinding interaction studies (e.g., SPR\/BLI or plate-based binding formats)\u003c\/li\u003e\n\u003cli\u003eCell-based stimulation studies with downstream marker readouts (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P04626 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P04626 - NCBI Gene search: ERBB2 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ERBB2 - Ensembl search: ERBB2 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ERBB2 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053405069677,"sku":"CSB-EP007763HU1-1MG","price":1812.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053529948525,"sku":"CSB-EP007763HU1-100UG","price":419.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053529981293,"sku":"CSB-EP007763HU1-20UG","price":224.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP007763HU1-1.jpg?v=1772177644","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-receptor-tyrosine-protein-kinase-erbb-2-erbb2-partial-bhp10507183","provider":"BioHippo","version":"1.0","type":"link"}