{"product_id":"recombinant-human-robo1-protein-c-his-bhp21400001","title":"Recombinant Human ROBO1 Protein, C-His","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat is ROBO1?\u003c\/strong\u003e ROBO1 (Roundabout guidance receptor 1; also reported under aliases such as DUTT1\/SAX3 in some resources) encodes a \u003cstrong\u003ecell-surface, single-pass type I transmembrane receptor\u003c\/strong\u003e in the Roundabout (ROBO) family. It is a member of the \u003cstrong\u003eimmunoglobulin superfamily\u003c\/strong\u003e and functions as an \u003cstrong\u003eaxon guidance and cell migration receptor\n\n\u003cp\u003e\u003cstrong\u003eSubcellular localization (research context):\u003c\/strong\u003e ROBO1 is primarily associated with the \u003cstrong\u003eplasma membrane\u003c\/strong\u003e as a guidance receptor. Depending on cell type and experimental context, receptor trafficking and compartmental distribution can shift as part of receptor regulation and signaling-state changes.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eDomain \/ architecture (why fragments matter):\u003c\/strong\u003e ROBO receptors share a conserved ectodomain organization built for extracellular recognition. ROBO1 is commonly described as containing \u003cstrong\u003efive immunoglobulin-like domains\u003c\/strong\u003e and \u003cstrong\u003ethree fibronectin type III domains\u003c\/strong\u003e in its extracellular region, followed by a single transmembrane segment and a cytoplasmic signaling region in the full-length receptor.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat this recombinant protein represents:\u003c\/strong\u003e This product is a \u003cstrong\u003erecombinant human ROBO1 protein fragment\u003c\/strong\u003e corresponding to \u003cstrong\u003eGln26–Pro897\u003c\/strong\u003e (a large portion of the extracellular region), expressed in \u003cstrong\u003emammalian cells\u003c\/strong\u003e and supplied as a purified, soluble reagent with a \u003cstrong\u003eC-terminal His tag\u003c\/strong\u003e. As a soluble ectodomain reagent, it is typically used when researchers want a \u003cstrong\u003edefined ROBO1 input\u003c\/strong\u003e for controlled binding\/interaction studies, benchmarking reagents, or building reproducible assay systems where endogenous ROBO1 is variable by cell type, isoform, and expression level.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eCore pathway identity:\u003c\/strong\u003e ROBO1 is best known as a receptor in the \u003cstrong\u003eSLIT–ROBO signaling axis\u003c\/strong\u003e. In classic guidance biology, ROBO1 helps cells and axons interpret extracellular cues during nervous system development and in migration-related paradigms.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eLigand-driven guidance signaling (research framing):\u003c\/strong\u003e ROBO1 is activated by \u003cstrong\u003eSLIT-family ligands\u003c\/strong\u003e, producing guidance outcomes that are often described as repulsive in axon pathfinding systems. In broader experimental contexts, SLIT–ROBO outputs can be strongly dependent on cell type, receptor composition, and the local signaling environment.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhy researchers study ROBO1:\u003c\/strong\u003e ROBO1 links \u003cstrong\u003eextracellular cue recognition\u003c\/strong\u003e to \u003cstrong\u003ecytoskeletal and polarity control\u003c\/strong\u003e, making it a common entry point for studying directional migration and guidance decisions. Functionally, ROBO-family signaling is frequently discussed alongside downstream regulators that shape actin dynamics and cell movement behavior, supporting mechanistic hypotheses about how guidance receptors alter protrusion, adhesion, and directional persistence.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eDevelopmental neurobiology and beyond:\u003c\/strong\u003e While SLIT–ROBO biology is strongly associated with nervous system wiring and midline guidance, the pathway is broadly used in research settings that examine cell positioning and directed migration. Recombinant ROBO1 ectodomain reagents are therefore useful when the experimental goal is to isolate ligand recognition and binding specificity from intracellular signaling complexity.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eMolecular characteristics relevant to experimental design\u003c\/h2\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein class:\u003c\/strong\u003e Single-pass type I membrane receptor (full-length); this product corresponds to a \u003cstrong\u003esoluble extracellular-region fragment\u003c\/strong\u003e (Gln26–Pro897).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDomain logic:\u003c\/strong\u003e The ROBO ectodomain is built from repeated recognition modules (Ig-like and FnIII domains), which are commonly discussed as key structural elements supporting ligand engagement and receptor organization.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eLocalization implication:\u003c\/strong\u003e Because ROBO1 is primarily associated with the \u003cstrong\u003eplasma membrane\u003c\/strong\u003e in cells, soluble ectodomain reagents are typically used to focus on \u003cstrong\u003eextracellular recognition\u003c\/strong\u003e and binding determinants, separate from membrane trafficking and cytosolic adaptor recruitment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cstrong\u003ePost-translational modification considerations:\u003c\/strong\u003e ROBO1 is an extracellular, multi-domain receptor; for many cell-surface receptors of this class, native-like folding and glycosylation can influence conformation, epitope accessibility, and ligand binding behavior. Mammalian expression is commonly selected to better preserve these extracellular features compared with purely prokaryotic expression systems.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eExpression and purification context (quality transparency for RUO)\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e Mammalian cells.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eRegion expressed:\u003c\/strong\u003e Gln26–Pro897 (soluble ectodomain fragment), with \u003cstrong\u003eC-His\u003c\/strong\u003e tag.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity chromatography; reported purity \u0026gt;90% (per your product data).\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eFormulation note (interpretation):\u003c\/strong\u003e Stabilizing buffer systems and lyophilized formats are commonly used for recombinant ectodomains to support storage stability and reduce aggregation risk. (Operational handling and shipping details should remain in your separate “Available Options \/ Storage \u0026amp; Shipping” fields, not here.)\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eHow to think about ROBO1 ectodomain reagents in research\u003c\/h2\u003e\n\n\u003cp\u003eFor many receptor systems, the ectodomain is where experimentally tractable questions begin: \u003cstrong\u003ewhat binds\u003c\/strong\u003e, \u003cstrong\u003ewith what specificity\u003c\/strong\u003e, and \u003cstrong\u003ehow domain composition shapes recognition\u003c\/strong\u003e. A recombinant ROBO1 extracellular fragment can serve as a \u003cstrong\u003edefined molecular reference\u003c\/strong\u003e for comparing binding behavior across reagents and experimental systems.\u003c\/p\u003e\n\n\u003cp\u003eThis can be especially helpful when results need to be compared across cell lines or engineered models where endogenous ROBO1 abundance, isoform usage, and membrane organization differ, and where intracellular adaptor availability adds additional variability to full-length receptor signaling.\u003c\/p\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 ug","offer_id":53000605434221,"sku":"HP485011-100UG","price":478.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53000605466989,"sku":"HP485011-1MG","price":2878.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-robo1-protein-c-his-bhp21400001","provider":"BioHippo","version":"1.0","type":"link"}