{"product_id":"recombinant-human-schlafen-family-member-12-like-slfn12l-bhp10505922","title":"Recombinant Human Schlafen family member 12-like (SLFN12L)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Homo sapiens (Human): Human Schlafen family member 12-like (SLFN12L) corresponding to amino acids 1–588. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–588 (588 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 1TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt Q6IEE8. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman Schlafen family member 12-like (SLFN12L) is a member of the Schlafen (SLFN) family, a group of proteins frequently discussed in the context of interferon responses, immune-cell regulation, and antiviral restriction. Family members differ in domain composition and reported cellular roles. For less-characterized SLFN paralogs, sequence-defined recombinant constructs are often used to support antibody development and domain-focused biochemical studies. Also reported as: SLFN12L; SLFN5Schlafen family member 12-like.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eInterferon-stimulated gene (ISG) network mapping to define when and where SLFN paralogs are induced and what pathways they influence.\u003c\/li\u003e\n\u003cli\u003eMechanistic studies of SLFN-associated effects on RNA metabolism, translation, or cell differentiation (varies by paralog).\u003c\/li\u003e\n\u003cli\u003eComparative functional genomics to separate conserved versus lineage-specific SLFN activities.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReagent development (antibodies\/binders) to track SLFN expression and subcellular distribution.\u003c\/li\u003e\n\u003cli\u003eDomain-centric interaction studies to identify binding partners and infer pathway placement.\u003c\/li\u003e\n\u003cli\u003eComparative studies across species or paralogs to interpret conserved motifs and potential functional divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human Schlafen family member 12-like (SLFN12L) (Q6IEE8) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q6IEE8\/entry\n- NCBI Gene search: SLFN12L Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLFN12L%20Homo%20sapiens\n- PubMed search: SLFN12L review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLFN12L%20review\n- InterPro search: SLFN12L — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/SLFN12L\/\n- Ensembl Gene Summary: SLFN12L (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=SLFN12L\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207324000621,"sku":"CSB-CF021762HU-100UG","price":2781.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320591540589,"sku":"CSB-CF021762HU-20UG","price":1668.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF021762HU-SDS.jpg?v=1778623123","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-schlafen-family-member-12-like-slfn12l-bhp10505922","provider":"BioHippo","version":"1.0","type":"link"}