{"product_id":"recombinant-human-signal-recognition-particle-54kda-bhp11004866","title":"Recombinant Human Signal Recognition Particle 54kDa","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Human Signal Recognition Particle 54kDa\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eSRP54 is a 54-kDa subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex which leads the translocation of newly synthesized secretory proteins from the polysome to the endoplasmic reticulum. SRP is composed of six polypeptides and a tRNA-like molecule known as 7SL RNA. SRP54 is a GTP-binding protein that directly binds the signal sequences of nascent secretory and membrane proteins. Anti-SRP autoantibodies take place in patients with an autoimmune chronic muscle inflammation called polymyositis. These auto-antibodies predominantly recognize the SRP54 subunit and in addition are able to immunoprecipitate several of the SRP subunits and the RNA component. Nearly 5% of myositis patients are positive for anti-SRP autoantibodies, increasing to 18% in the subgroup of Jo-1 autoantibody-negative patients. The classic 'anti-SRP syndrome' is a severe form of polymyositis in which the myositic inflammation is acute and aggressive onset, with common myalgias and cardiac involvement. Normally there is a poor reaction to therapy with a 5-year survival rate of about 25%.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunological functions:\u003c\/strong\u003e 1. Binds IgG-type human auto-antibodies.\u003cbr\u003e2. Standard ELISA test (checkerboard analysis of positive\/negative sera panels); immunodot test with positive\/negative sera panels.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoating concentration:\u003c\/strong\u003e 0.5-0.9 µg\/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"5 ug","offer_id":53038159921517,"sku":"pro-113-5UG","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53038499266925,"sku":"pro-113-20UG","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038499299693,"sku":"pro-113-1MG","price":3900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-srp54-human-PRO-113.png?v=1782158349","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-signal-recognition-particle-54kda-bhp11004866","provider":"BioHippo","version":"1.0","type":"link"}