{"product_id":"recombinant-human-smad4-protein-his-tag-bhp10304058","title":"Recombinant Human SMAD4 Protein (His Tag)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-right:8px}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eSMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin\/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2\/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2\/SMAD4\/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative names\u003c\/strong\u003e: DPC4;Deletion Target in Pancreatic Carcinoma 4;MAD Homolog 4;MADH4;Mothers Against DPP Homolog 4;Mothers Against Decapentaplegic Homolog 4;SMAD 4;SMAD Family Member 4;SMAD4;Smad4;hSMAD4\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eE. coli Expression\u003c\/span\u003e\n\u003cp\u003eThis recombinant protein is produced using a validated E. coli expression system and supplied as lyophilized powder with C-His affinity tag. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n  \u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eQ13485\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression Region\u003c\/th\u003e\n\u003ctd\u003eMet 1-Asp552\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eAffinity Tag\u003c\/th\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eCalculated MW\u003c\/th\u003e\n\u003ctd\u003e61.5 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eObserved MW\u003c\/th\u003e\n\u003ctd\u003e62 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 80% as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eEndotoxin\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.0.\u003cbr\u003eNormal…\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eGenerally, lyophilized proteins are stable for up to 12 months when stored at -2…\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003cdiv class=\"bhp-qc-badge\"\u003e✓ Purity Verified by SDS-PAGE\u003c\/div\u003e \u003cdiv class=\"bhp-qc-badge\"\u003e✓ LAL Endotoxin-Tested\u003c\/div\u003e\n\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp\u003eSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology Co Ltd","offers":[{"title":"10 ug","offer_id":53326493778285,"sku":"PKSH033066_10μg","price":88.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53326512226669,"sku":"PKSH033066_50μg","price":198.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4lHpSC.jpg?v=1778704767","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-smad4-protein-his-tag-bhp10304058","provider":"BioHippo","version":"1.0","type":"link"}