{"product_id":"recombinant-human-sonic-hedgehog-shh-bhp11300459","title":"Recombinant Human Sonic HedgeHog (Shh)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-right:8px}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eMembers of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule.\u003c\/p\u003e\n\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eE. coli Expression\u003c\/span\u003e\n\u003cp\u003eRecombinant Sonic HedgeHog Human protein is produced using a validated E. coli expression system and supplied as lyophilized powder for long-term stability. Suitable for use in functional bioassays, ELISA standard curves, receptor binding studies, antibody validation, and related research applications.\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;98% by SDS-PAGE and HPLC analyses.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eEndotoxin\u003c\/th\u003e\n\u003ctd\u003eLess than 1EU\/µg of rHuSHH as determined by LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003ePhysical Form\u003c\/th\u003e\n\u003ctd\u003eSterile Filtered White lyophilized (freeze-dried) powder.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 150m…\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eThis lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, …\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003cdiv class=\"bhp-qc-badge\"\u003e✓ Purity Verified by SDS-PAGE\u003c\/div\u003e \u003cdiv class=\"bhp-qc-badge\"\u003e✓ LAL Endotoxin-Tested\u003c\/div\u003e\n\n\u003ch2\u003eFunctional Activity\u003c\/h2\u003e\n\u003cp\u003eDetermined by its ability to induce alkaline phosphatase production by C3H\/10T1\/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg\/ml, corresponding to a Specific Activity of ＞1 x 103 IU\/mg.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eThis material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.   Made  in China\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"1 mg","offer_id":53326147453293,"sku":"PR1114-1MG","price":0.0,"currency_code":"USD","in_stock":true},{"title":"5 ug","offer_id":53326171406701,"sku":"PR1114-5UG","price":158.0,"currency_code":"USD","in_stock":true},{"title":"25 ug","offer_id":53326171439469,"sku":"PR1114-25UG","price":298.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-sonic-hedgehog-shh-bhp11300459","provider":"BioHippo","version":"1.0","type":"link"}