{"product_id":"recombinant-human-sonic-hedgehog-shh-protein-bhp10304475","title":"Recombinant Human Sonic Hedgehog\/SHH Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Sonic Hedgehog\/SHH Protein\u003c\/strong\u003e — also known as HHG-1; SHH; Sonic Hedgehog Protein — Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems; such as limbs and brain. SHH also plays an important role in adult; including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor; and undergoes a series of processi\u003c\/p\u003e\n\u003cp\u003eResearch into Human Sonic Hedgehog\/SHH Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-cat-pill\"\u003eE. coli (Prokaryotic)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eProduced in \u003cstrong\u003eE. coli (Prokaryotic)\u003c\/strong\u003e, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.\u003c\/p\u003e\n\u003cp\u003eExpression region: \u003cstrong\u003eCys24-Gly197\u003c\/strong\u003e.\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight (Calculated)\u003c\/th\u003e\n\u003ctd\u003e19.7 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight (Observed SDS-PAGE)\u003c\/th\u003e\n\u003ctd\u003e19 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 95% as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eE. coli (Prokaryotic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 0.01 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refe\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003ePlease refer to the printed manual for detailed information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003eGenerally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Lead Time\u003c\/th\u003e\n\u003ctd\u003e2-3 business days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession Number\u003c\/th\u003e\n\u003ctd\u003eQ15465\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eQ15465\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression Region\u003c\/th\u003e\n\u003ctd\u003eCys24-Gly197\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003ePurity \u0026gt;95%\u003c\/span\u003e \u003cspan class=\"bhp-qc-badge\"\u003eEndotoxin Tested\u003c\/span\u003e\n\u003ch2\u003eBiological Activity\u003c\/h2\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003eActivity Validated\u003c\/span\u003e\n\u003cp\u003eNot validated for activity\u003c\/p\u003e\n\u003cp\u003ePerform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eElabscience Biotechnology Co Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003ePKSH033519\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003eHuman\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eUniProt\u003c\/strong\u003eQ15465\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology Co Ltd","offers":[{"title":"10 ug","offer_id":53335891935597,"sku":"PKSH033519_10μg","price":88.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53335893508461,"sku":"PKSH033519_50μg","price":198.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/0GK0pL.png?v=1778912994","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-sonic-hedgehog-shh-protein-bhp10304475","provider":"BioHippo","version":"1.0","type":"link"}