{"product_id":"recombinant-human-sparc-protein-sparc-partial-active-bhp10506029","title":"Recombinant Human SPARC protein (SPARC), partial (Active)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eSPARC\u003c\/strong\u003e from Homo sapiens (Human), produced in E.Coli (region 18-303aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 18-303aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.Coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Basement-membrane protein 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine,. Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving SPARC\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P09486 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P09486 - NCBI Gene search: SPARC — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPARC - Ensembl search: SPARC — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPARC - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"500 ug","offer_id":53053374497133,"sku":"CSB-AP000301HU-500UG","price":1308.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053468606829,"sku":"CSB-AP000301HU-100UG","price":584.0,"currency_code":"USD","in_stock":true},{"title":"10 ug","offer_id":53053468639597,"sku":"CSB-AP000301HU-10UG","price":142.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-AP000301HU.jpg?v=1772177531","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-sparc-protein-sparc-partial-active-bhp10506029","provider":"BioHippo","version":"1.0","type":"link"}