{"product_id":"recombinant-human-steap-family-member-1b-steap1b-bhp10510504","title":"Recombinant Human STEAP family member 1B (STEAP1B)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Homo sapiens (Human): Human STEAP family member 1B (STEAP1B) corresponding to amino acids 1–245. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–245 (245 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 4TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt Q6NZ63. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman STEAP family member 1B (STEAP1B) is a member of the STEAP family, a group of multi-pass membrane proteins that have been reported to participate in metal ion reduction\/transport-related processes and are commonly studied as cell-surface antigens in certain research contexts. When presented on virus-like particles (VLPs), multi-pass membrane proteins can retain membrane topology and conformational epitopes that are difficult to reproduce with soluble fragments.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-surface proteomics and antigen discovery efforts that profile STEAP-family expression across tissues and model systems (RUO).\u003c\/li\u003e\n\u003cli\u003eDevelopment of binding reagents (antibodies, nanobodies) that recognize conformational epitopes on native-like membrane presentations.\u003c\/li\u003e\n\u003cli\u003eMechanistic studies of STEAP-associated redox chemistry and its coupling to membrane transport processes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBinder screening against VLP-displayed antigen to enrich for conformation-sensitive reagents.\u003c\/li\u003e\n\u003cli\u003eAssay development for detecting cell-surface STEAP-family proteins in engineered cell models (RUO).\u003c\/li\u003e\n\u003cli\u003eFunctional studies exploring how perturbing STEAP expression affects cellular metal handling and redox phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human STEAP family member 1B (STEAP1B) (Q6NZ63) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q6NZ63\/entry\n- NCBI Gene search: STEAP1B Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STEAP1B%20Homo%20sapiens\n- PubMed search: STEAP1B review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STEAP1B%20review\n- InterPro search: STEAP1B — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/STEAP1B\/\n- Ensembl Gene Summary: STEAP1B (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=STEAP1B\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207329407341,"sku":"CSB-CF754160HU-100UG","price":1751.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320597897581,"sku":"CSB-CF754160HU-20UG","price":1071.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF754160HU-SDS.jpg?v=1778623134","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-steap-family-member-1b-steap1b-bhp10510504","provider":"BioHippo","version":"1.0","type":"link"}