{"product_id":"recombinant-human-thrombin-hek-bhp11002693","title":"Recombinant Human Thrombin, HEK","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Human Thrombin, HEK\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eThrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Conditions:\u003c\/strong\u003e Thrombin (1nM) was assayed using SPECTROZYME TH as a substrate (20µM) in 5mM Tris-HCl (pH8.0), 0.1% PEG, 200mM NaCl at 25°C.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"10 ug","offer_id":53038088749421,"sku":"pro-1422-10UG","price":200.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53038361117037,"sku":"pro-1422-50UG","price":750.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53038361149805,"sku":"pro-1422-100UG","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-thrombin-human-recombinant-PRO-1422.png?v=1782158209","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-thrombin-hek-bhp11002693","provider":"BioHippo","version":"1.0","type":"link"}