{"product_id":"recombinant-human-transcription-factor-mafk-mafk-bhp10505808","title":"Recombinant Human Transcription factor MafK (MAFK)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eMAFK\u003c\/strong\u003e from Homo sapiens (Human), produced in Baculovirus (region 1-156aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-156aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e Baculovirus (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, Myc (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Erythroid transcription factor NF-E2 p18 subunit. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eSince they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving MAFK\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB O60675 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/O60675 - NCBI Gene search: MAFK — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAFK - Ensembl search: MAFK — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAFK - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053369778541,"sku":"CSB-BP013321HU-1MG","price":2464.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053460283757,"sku":"CSB-BP013321HU-100UG","price":986.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053460316525,"sku":"CSB-BP013321HU-20UG","price":352.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-BP013321HU-SDS.jpg?v=1772177517","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-transcription-factor-mafk-mafk-bhp10505808","provider":"BioHippo","version":"1.0","type":"link"}