{"product_id":"recombinant-human-transcriptional-enhancer-factor-tef-3-tead4-partial-bhp10507570","title":"Recombinant Human Transcriptional enhancer factor TEF-3 (TEAD4), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eTEAD4\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 74-434aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 74-434aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e GST (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as TEA domain family member 4 (TEAD-4) (Transcription factor 13-like 1) (Transcription factor RTEF-1) (RTEF1) (TCF13L1) (TEF3). Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1\/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1\/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1\/TAZ. Acts by mediating gene expression of YAP1 and WWTR1\/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons'and activates transcription. Binds to the M-CAT motif.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1\/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1\/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1\/TAZ. Acts by mediating gene expression of YAP1 and WWTR1\/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons'and activates transcription. Binds to the M-CAT motif.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving TEAD4\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q15561 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q15561 - NCBI Gene search: TEAD4 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TEAD4 - Ensembl search: TEAD4 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TEAD4 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053416964461,"sku":"CSB-EP618010HUe0-1MG","price":1812.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053555507565,"sku":"CSB-EP618010HUe0-100UG","price":419.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053555540333,"sku":"CSB-EP618010HUe0-20UG","price":224.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP618010HUe0-SDS.jpg?v=1772177686","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-transcriptional-enhancer-factor-tef-3-tead4-partial-bhp10507570","provider":"BioHippo","version":"1.0","type":"link"}