{"product_id":"recombinant-human-tumor-necrosis-factor-ligand-superfamily-member-10-tnfsf10-partial-active-bhp10506715","title":"Recombinant Human Tumor necrosis factor ligand superfamily member 10 (TNFSF10), partial (Active)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eTNFSF10\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 115-281aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 115-281aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL. Cytokine that binds to TNFRSF10A\/TRAILR1, TNFRSF10B\/TRAILR2, TNFRSF10C\/TRAILR3, TNFRSF10D\/TRAILR4 and possibly also to TNFRSF11B\/OPG.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eHuman TNFSF10 is a type II transmembrane protein with an intracellular N-terminus and a ‘TNF homology domain’ (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. Two of these receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2\/TRICK2), are plasma membrane proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving TNFSF10\u003c\/li\u003e\n\u003cli\u003eBinding interaction studies (e.g., SPR\/BLI or plate-based binding formats)\u003c\/li\u003e\n\u003cli\u003eCell-based stimulation studies with downstream marker readouts (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P50591 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P50591 - NCBI Gene search: TNFSF10 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TNFSF10 - Ensembl search: TNFSF10 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TNFSF10 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053391372653,"sku":"CSB-AP004901HU-1MG","price":1702.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53053504455021,"sku":"CSB-AP004901HU-500UG","price":1190.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53053504487789,"sku":"CSB-AP004901HU-50UG","price":270.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-AP004901HU.jpg?v=1772177596","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-tumor-necrosis-factor-ligand-superfamily-member-10-tnfsf10-partial-active-bhp10506715","provider":"BioHippo","version":"1.0","type":"link"}