{"product_id":"recombinant-human-urea-transporter-slc14a1-bhp10510824","title":"Recombinant Human Urea transporter (SLC14A1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Homo sapiens (Human): Human Urea transporter (SLC14A1) corresponding to amino acids 1–389. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–389 (389 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 8TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt A0A7L9QHR7. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman Urea transporter (SLC14A1) is annotated as a urea transporter in the SLC14 family. Urea transporters are multi-pass membrane proteins that facilitate urea movement across membranes, supporting osmotic balance and nitrogen handling in tissues where urea gradients are physiologically important. Studies often focus on transporter expression patterns, membrane localization, and how sequence variants influence transport properties.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIntegrating transcriptomics\/proteomics to refine where urea transporters are expressed and how they respond to osmotic or hormonal cues.\u003c\/li\u003e\n\u003cli\u003eStructure–function mapping of transmembrane segments and extracellular loops that contribute to selectivity and inhibition.\u003c\/li\u003e\n\u003cli\u003eVariant interpretation and genotype–phenotype research in model systems (RUO).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntibody development and detection studies targeting extracellular or cytosolic regions of SLC14A transporters.\u003c\/li\u003e\n\u003cli\u003eComparative analysis of transporter variants\/orthologs to probe conserved motifs.\u003c\/li\u003e\n\u003cli\u003eInteraction studies with scaffolding proteins or lipids that may affect surface stability and trafficking.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human Urea transporter (SLC14A1) (A0A7L9QHR7) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/A0A7L9QHR7\/entry\n- NCBI Gene search: SLC14A1 Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLC14A1%20Homo%20sapiens\n- PubMed search: SLC14A1 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLC14A1%20review\n- InterPro search: SLC14A1 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/SLC14A1\/\n- Ensembl Gene Summary: SLC14A1 (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=SLC14A1\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207329866093,"sku":"CSB-CF4165HU-100UG","price":1751.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320598421869,"sku":"CSB-CF4165HU-20UG","price":1071.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF4165HU-SDS.jpg?v=1778623136","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-urea-transporter-slc14a1-bhp10510824","provider":"BioHippo","version":"1.0","type":"link"}