{"product_id":"recombinant-lasv-nucleoprotein-protein-n-his-c-his-bhp21414359","title":"Recombinant LASV Nucleoprotein Protein, N-His \u0026 C-His","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Viral Protein\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant LASV Nucleoprotein Protein, N-His \u0026amp; C-His\u003c\/strong\u003e is a recombinant \u003cstrong\u003enucleoprotein (NP) of Lassa virus\u003c\/strong\u003e, expressed in \u003cem\u003eE. coli\u003c\/em\u003e with a His tag at each terminus. The manufacturer gives the expressed range as \u003cstrong\u003eMet1-Leu569\u003c\/strong\u003e. It is supplied lyophilized at \u0026gt;90% purity as determined by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eThe manufacturer lists ELISA, use as an immunogen, SDS-PAGE and Western blot as applications. Bioactivity testing is stated by the manufacturer as in progress, so no functional activity data are provided for this product. No predicted molecular weight is stated for this product; confirm the band size on your own SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: E. coli · Tag: N-His \u0026amp; C-His\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eLassa virus (LASV) is a mammarenavirus and the agent of Lassa fever, which is endemic in parts of West Africa. Its genome is divided between two ambisense RNA segments: the S segment encodes the nucleoprotein and the glycoprotein precursor, and the L segment encodes the polymerase and the Z protein. Recombinant LASV proteins are used as coating antigens in ELISA, as immunogens for antibody production, and as reference material in SDS-PAGE and Western blot.\u003c\/p\u003e\n\u003cp\u003eThe nucleoprotein is the most abundant protein in arenavirus particles and in infected cells. It coats the viral RNA to form the ribonucleoprotein complex that the polymerase uses as its template. Because it is abundant and strongly immunogenic, arenavirus nucleoprotein is a common antigen in serological assays and a frequent immunogen for raising detection antibodies.\u003c\/p\u003e\n\u003ch2\u003eAbout the Construct\u003c\/h2\u003e\n\u003cp\u003eThe expressed range is given by the manufacturer as \u003cstrong\u003eMet1-Leu569\u003c\/strong\u003e, numbered on UniProt accession P13699. Use that accession as the reference when comparing this product with the same target from another supplier. The His tags are additional sequence at each terminus and are not part of the stated range.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eNucleoprotein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eLassa virus (LASV)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eN-His \u0026amp; C-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eMet1-Leu569\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eP13699\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePredicted molecular weight\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. The stock concentration is set by the volume used for reconstitution.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of the datasheet.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Immunogen, SDS-PAGE, Western Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBioactivity\u003c\/th\u003e\n\u003ctd\u003eTesting in progress (manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eNucleocapsid protein, Protein N, N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eReconstitute in sterile water for a stock solution. The manufacturer does not state a concentration for this product, so choose the volume of sterile water that gives the stock concentration your assay needs. If the product label or datasheet gives a recommended value, follow that instead.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution protocol\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eLet the product and the reconstitution diluent reach room temperature.\u003c\/li\u003e\n\u003cli\u003eTap or briefly centrifuge the vial before opening to bring down any lyophilized material on the wall or cap.\u003c\/li\u003e\n\u003cli\u003eIf the product datasheet gives a recommended diluent and stock concentration, follow it. If you need a more concentrated stock than it recommends, contact us.\u003c\/li\u003e\n\u003cli\u003eAfter adding the diluent, re-cap the vial and invert gently by hand, or place it on a slow rocking platform, so the diluent coats all inner surfaces. \u003cem\u003eDo not mix by vortexing or by pipetting up and down.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003eLet the vial sit at room temperature with gentle agitation for at least 15 minutes before aliquoting or use.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted material in single-use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and storage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eIn general, proteins are provided as lyophilized powder\/frozen liquid. They are shipped out with dry ice\/blue ice unless customers require otherwise.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eThis product is a recombinant protein expressed in \u003cem\u003eE. coli\u003c\/em\u003e; it contains no live or inactivated virus. Handle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze-thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":67704163664237,"sku":"VK810032-50UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67704163697005,"sku":"VK810032-100UG","price":411.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":67704163729773,"sku":"VK810032-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Protein_Placeholder_Image_dfbcee2b-3aa3-45ae-82da-3faed2a3c337.jpg?v=1792184963","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-lasv-nucleoprotein-protein-n-his-c-his-bhp21414359","provider":"BioHippo","version":"1.0","type":"link"}