{"product_id":"recombinant-legionella-pneumophila-chaperonin-groel-groel-partial-bhp10508085","title":"Recombinant Legionella pneumophila Chaperonin GroEL (groEL), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003egroL\u003c\/strong\u003e from Legionella pneumophila, produced in E.coli (region 22-524aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 22-524aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as 58 kDa common antigen GroEL protein Heat shock protein B Protein Cpn60. Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. May play a protective role against the defense mechanisms generated by the infected macrophages.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003ePrevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. May play a protective role against the defense mechanisms generated by the infected macrophages.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving groL\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P69050 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P69050 - NCBI Gene search: groL — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=groL - Ensembl search: groL — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=groL - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053432889709,"sku":"CSB-EP302430LNY-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053587816813,"sku":"CSB-EP302430LNY-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053587849581,"sku":"CSB-EP302430LNY-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP302430LNY-SDS.jpg?v=1772177744","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-legionella-pneumophila-chaperonin-groel-groel-partial-bhp10508085","provider":"BioHippo","version":"1.0","type":"link"}