{"product_id":"recombinant-macroh2a-1-antibody-h2afy-bha17103733","title":"Recombinant MacroH2A.1 Antibody \/ H2AFY","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant MacroH2A.1 antibody supplied as a purified reagent for WB, ELISA, ICC in Human samples. This product is a recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Recombinant Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM248.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, ELISA, ICC.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A peptide corresponding to the C-terminus of human Histone macroH2A1 was used as the immunogen for this recombinant MacroH2A.1 antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eRecombinant MacroH2A.1 is the intended antigen for this primary antibody. Reported biological context includes: This recombinant MacroH2A.1 antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eELISA: support quantitative detection workflows and assay development where a defined antibody reagent is needed for capture or detection.\u003c\/li\u003e   \u003cli\u003eImmunocytochemistry (ICC): visualize intracellular distribution and morphology-linked changes in cultured cells.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (Q9QZQ8) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/Q9QZQ8\/entry - NCBI Gene search (Recombinant MacroH2A.1) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Recombinant+MacroH2A.1 - Ensembl search (Recombinant MacroH2A.1) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Recombinant+MacroH2A.1 - PubMed search (Recombinant MacroH2A.1) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Recombinant+MacroH2A.1 - Reactome pathway search (Recombinant MacroH2A.1) — Reactome — https:\/\/reactome.org\/content\/query?q=Recombinant+MacroH2A.1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ug","offer_id":53043248595309,"sku":"R20247-100UG","price":485.0,"currency_code":"USD","in_stock":true},{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 25 ug","offer_id":53043680706925,"sku":"R20247-25UG","price":259.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d95404b8-293e-4e05-badc-549bbf3bf746.jpg?v=1771934400","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-macroh2a-1-antibody-h2afy-bha17103733","provider":"BioHippo","version":"1.0","type":"link"}