{"product_id":"recombinant-macv-glycoprotein-g1-protein-c-his-bhp21414328","title":"Recombinant MACV Glycoprotein G1 Protein, C-His","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Viral Protein\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant MACV Glycoprotein G1 Protein, C-His\u003c\/strong\u003e is a recombinant \u003cstrong\u003eglycoprotein G1 of Machupo virus\u003c\/strong\u003e, expressed in \u003cem\u003emammalian cells\u003c\/em\u003e with a C-His tag. The manufacturer gives the expressed range as \u003cstrong\u003eGlu87-Leu261\u003c\/strong\u003e and a predicted molecular weight of 23.13 kDa. It is supplied lyophilized at \u0026gt;90% purity as determined by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eThe manufacturer lists ELISA, use as an immunogen, SDS-PAGE and Western blot as applications. Bioactivity testing is stated by the manufacturer as in progress, so no functional activity data are provided for this product.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Mammalian Cells · Tag: C-His\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eMachupo virus (MACV) is a mammarenavirus and the agent of Bolivian haemorrhagic fever. Its genome is divided between two ambisense RNA segments: the S segment encodes the nucleoprotein and the glycoprotein precursor, and the L segment encodes the polymerase and the Z protein. Recombinant MACV proteins are used as coating antigens in ELISA, as immunogens for antibody production, and as reference material in SDS-PAGE and Western blot.\u003c\/p\u003e\n\u003cp\u003eArenaviruses make their surface glycoproteins as a single precursor that is cut into a stable signal peptide and two subunits, G1 and G2. G1 sits on the outside of the particle and carries the receptor-binding function, while G2 is the transmembrane subunit that drives membrane fusion. Both subunits are produced separately as recombinant antigens for binding assays, antibody screening and immunisation.\u003c\/p\u003e\n\u003ch2\u003eAbout the Construct\u003c\/h2\u003e\n\u003cp\u003eThe expressed range is given by the manufacturer as \u003cstrong\u003eGlu87-Leu261\u003c\/strong\u003e, and the manufacturer cites RefSeq accession NP_899212.1, a glycoprotein precursor entry. The residue numbers refer to that entry, so compare them against the accession rather than against the protein counted from its own first residue. The C-His tag is added outside the stated range.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eGlycoprotein G1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eMachupo virus (MACV)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eMammalian Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein length\u003c\/th\u003e\n\u003ctd\u003eGlu87-Leu261\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eNP_899212.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePredicted molecular weight\u003c\/th\u003e\n\u003ctd\u003e23.13 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Immunogen, SDS-PAGE, Western Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBioactivity\u003c\/th\u003e\n\u003ctd\u003eTesting in progress (manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eGlycoprotein precursor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eReconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution protocol\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eLet the product and the reconstitution diluent reach room temperature.\u003c\/li\u003e\n\u003cli\u003eTap or briefly centrifuge the vial before opening to bring down any lyophilized material on the wall or cap.\u003c\/li\u003e\n\u003cli\u003eUse the diluent and stock concentration recommended in the product datasheet. If you need a stock concentration higher than recommended, contact us.\u003c\/li\u003e\n\u003cli\u003eAfter adding the diluent, re-cap the vial and invert gently by hand, or place it on a slow rocking platform, so the diluent coats all inner surfaces. \u003cem\u003eDo not mix by vortexing or by pipetting up and down.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003eLet the vial sit at room temperature with gentle agitation for at least 15 minutes before aliquoting or use.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted material in single-use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and storage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eIn general, proteins are provided as lyophilized powder\/frozen liquid. They are shipped out with dry ice\/blue ice unless customers require otherwise.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze-thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":67656813085037,"sku":"VK765021-50UG","price":398.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67656813314413,"sku":"VK765021-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":67656813347181,"sku":"VK765021-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VK765021-SDS-PAGE-1.jpg?v=1791814839","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-macv-glycoprotein-g1-protein-c-his-bhp21414328","provider":"BioHippo","version":"1.0","type":"link"}