{"product_id":"recombinant-macv-nucleoprotein-protein-n-his-bhp21414327","title":"Recombinant MACV Nucleoprotein Protein, N-His","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Viral Protein\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant MACV Nucleoprotein Protein, N-His\u003c\/strong\u003e is a recombinant \u003cstrong\u003enucleoprotein (NP) of Machupo virus\u003c\/strong\u003e, expressed in \u003cem\u003eE. coli\u003c\/em\u003e with an N-His tag. The manufacturer gives the expressed range as \u003cstrong\u003eMet1-Leu564\u003c\/strong\u003e and a predicted molecular weight of 65.46 kDa. It is supplied lyophilized at \u0026gt;90% purity as determined by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eThe manufacturer lists ELISA, use as an immunogen, SDS-PAGE and Western blot as applications. Bioactivity testing is stated by the manufacturer as in progress, so no functional activity data are provided for this product.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: E. coli · Tag: N-His\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eMachupo virus (MACV) is a mammarenavirus and the agent of Bolivian haemorrhagic fever. Its genome is divided between two ambisense RNA segments: the S segment encodes the nucleoprotein and the glycoprotein precursor, and the L segment encodes the polymerase and the Z protein. Recombinant MACV proteins are used as coating antigens in ELISA, as immunogens for antibody production, and as reference material in SDS-PAGE and Western blot.\u003c\/p\u003e\n\u003cp\u003eThe nucleoprotein is the most abundant protein in arenavirus particles and in infected cells. It coats the viral RNA to form the ribonucleoprotein complex that the polymerase uses as its template. Because it is abundant and strongly immunogenic, arenavirus nucleoprotein is a common antigen in serological assays and a frequent immunogen for raising detection antibodies.\u003c\/p\u003e\n\u003ch2\u003eAbout the Construct\u003c\/h2\u003e\n\u003cp\u003eThe expressed range is given by the manufacturer as \u003cstrong\u003eMet1-Leu564\u003c\/strong\u003e, and the manufacturer cites UniProt accession P26578 for this protein. The residue numbers refer to that entry, so compare them against the accession rather than against the protein counted from its own first residue. The N-His tag is added outside the stated range.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eNucleoprotein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eMachupo virus (MACV)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eN-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein length\u003c\/th\u003e\n\u003ctd\u003eMet1-Leu564\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eP26578\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePredicted molecular weight\u003c\/th\u003e\n\u003ctd\u003e65.46 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Immunogen, SDS-PAGE, Western Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBioactivity\u003c\/th\u003e\n\u003ctd\u003eTesting in progress (manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eNucleocapsid protein, Protein N, N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eReconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution protocol\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eLet the product and the reconstitution diluent reach room temperature.\u003c\/li\u003e\n\u003cli\u003eTap or briefly centrifuge the vial before opening to bring down any lyophilized material on the wall or cap.\u003c\/li\u003e\n\u003cli\u003eUse the diluent and stock concentration recommended in the product datasheet. If you need a stock concentration higher than recommended, contact us.\u003c\/li\u003e\n\u003cli\u003eAfter adding the diluent, re-cap the vial and invert gently by hand, or place it on a slow rocking platform, so the diluent coats all inner surfaces. \u003cem\u003eDo not mix by vortexing or by pipetting up and down.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003eLet the vial sit at room temperature with gentle agitation for at least 15 minutes before aliquoting or use.\u003c\/li\u003e\n\u003cli\u003eStore the reconstituted material in single-use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and storage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eIn general, proteins are provided as lyophilized powder\/frozen liquid. They are shipped out with dry ice\/blue ice unless customers require otherwise.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze-thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":67656813150573,"sku":"VK765012-50UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67656813478253,"sku":"VK765012-100UG","price":411.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":67656813511021,"sku":"VK765012-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VK765012-SDS-PAGE-1.jpg?v=1791814839","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-macv-nucleoprotein-protein-n-his-bhp21414327","provider":"BioHippo","version":"1.0","type":"link"}