{"product_id":"recombinant-micrurus-tener-tener-kunitz-type-neurotoxin-mittx-alpha-bhp10504229","title":"Recombinant Micrurus tener tener Kunitz-type neurotoxin MitTx-alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Micrurus tener tener Kunitz-type neurotoxin MitTx-alpha is a recombinant protein reagent for research-use applications such as assay development, binding studies, and mechanistic experiments. It corresponds to \u003cstrong\u003eMitTx-a\u003c\/strong\u003e (Micrurus tener tener (Texas coral snake)) and is intended for RUO workflows where a defined protein standard or functional input is needed.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (expression context can influence folding and PTMs).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e 25-84aa (region choice can affect activity and binding readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate(s)\/tag:\u003c\/strong\u003e N-terminal 6xHis-SUMO-tagged (can support detection or purification depending on format).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 23.1 kDa (useful for interpreting gel migration and size-exclusion profiles).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eWhen comparing results across assays, consider that expression system and expressed region can alter glycosylation, disulfide formation, and oligomerization state, which may shift apparent potency or binding behavior in vitro.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThis heterodimeric toxin potently activates mouse acid-sensing ion channel ASIC1\/ACCN2 expressed in Xenopus oocytes. Both alternatively spliced isoforms ASIC1a and ASIC1b are activated, with a higher potency for ASIC1a (EC(50)=9.4 nM) vs ASIC1b (EC(50)=23 nM). The ASIC3\/ACCN3 subtype is also sensitive to the heterodimer, but with a lower potency (EC(50)=830 nM). On ASIC2a\/ACCN1, the toxin shows a very weak activation, but produces a rarkable potentiation (\u0026gt;100-fold) of protons when the Extracellular domain pH drops below neutrality. The toxin interacts with the Extracellular domain region of the channel, since responses are only observed in the outside-out configuration. In vivo, the heterodimer elicits robust pain-related behavior in mice by activation of ASIC1\/ACCN2 channels on capsaicin-sensitive nerve fibers.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReagent standardization: using recombinant proteins as reference materials for quantitative calibration and cross-study comparability.\u003c\/li\u003e\n\u003cli\u003eInteraction-focused studies: mapping binding partners, affinity changes, and structure–function relationships across variants or domains.\u003c\/li\u003e\n\u003cli\u003eMulti-omic readouts: combining recombinant perturbations with transcript, protein, and functional endpoints to connect mechanism to phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssay development and validation: use as a defined input or standard where protein identity is required.\u003c\/li\u003e\n\u003cli\u003eBinding studies: evaluate interaction strength and specificity using plate-based or biophysical formats.\u003c\/li\u003e\n\u003cli\u003eCell-response profiling: add protein to cultured cells and interpret downstream marker changes with appropriate controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpretation is most robust when signal changes are evaluated relative to matched controls (buffer-only, unrelated protein controls, or pathway controls) and when readouts are compared across dose and time.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eIsoforms and PTMs can influence binding and activity; ensure the expressed region and expression system match your experimental needs.\u003c\/li\u003e\n\u003cli\u003eSpecies differences may affect receptor binding or antibody recognition; confirm species\/source alignment with your model.\u003c\/li\u003e\n\u003cli\u003eUse concept-level controls such as negative controls (no protein), matrix controls, or orthogonal readouts to support conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt keyword search: https:\/\/www.uniprot.org\/uniprotkb?query=MitTx-a - NCBI Gene search: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MitTx-a - PubMed search: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MitTx-a - Ensembl search: https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MitTx-a - Reactome Pathway Browser: https:\/\/reactome.org\/content\/query?q=MitTx-a --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053048947053,"sku":"CSB-EP516846MUA-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053200531821,"sku":"CSB-EP516846MUA-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053200564589,"sku":"CSB-EP516846MUA-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP516846MUA-SDS.jpg?v=1772172890","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-micrurus-tener-tener-kunitz-type-neurotoxin-mittx-alpha-bhp10504229","provider":"BioHippo","version":"1.0","type":"link"}