{"product_id":"recombinant-mouse-cxcl15-bhp10801014","title":"Recombinant Mouse Cxcl15","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eCxcl15\u003c\/strong\u003e is used in \u003cstrong\u003eresearch use only (RUO)\u003c\/strong\u003e settings as a defined recombinant protein reagent. Bench researchers commonly leverage recombinant proteins to create controlled experimental conditions for mechanistic studies, assay development, interaction mapping, and quantitative benchmarking across model systems.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Cxcl15, lungkine, Scyb15, weche.\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003eproteins like \u003cstrong\u003eCxcl15\u003c\/strong\u003e are studied as soluble signaling factors that coordinate cell–cell communication through receptor-mediated pathways. Researchers frequently use recombinant ligands to probe dose-responsive signaling programs, map receptor interactions, and benchmark functional assays in controlled experimental conditions.\u003c\/p\u003e\u003cp\u003eMechanistically, researchers often analyze how Cxcl15 participates in pathway networks through molecular interactions, localization, and regulated activity. Depending on the target class, this can involve receptor-mediated signaling, enzymatic catalysis, complex assembly, or structural organization that shapes downstream cellular phenotypes.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch relevance:\u003c\/strong\u003e RUO studies frequently connect Cxcl15 to perturbations such as immune stimulation, stress signaling, differentiation cues, metabolic remodeling, or engineered genetic modulation—then interpret downstream readouts using complementary pathway markers.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eMolecular features matter in RUO experiments:\u003c\/strong\u003e domain boundaries, oligomerization state, and PTM sensitivity can influence binding behavior, stability, and functional readouts in vitro.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Mouse\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConstruct \/ expression region:\u003c\/strong\u003e aa 26-167\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApprox. molecular weight:\u003c\/strong\u003e 37.0 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 90% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg\/ml by gently pipetting 2-3 times, don't vortex.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePTM considerations:\u003c\/strong\u003e For many extracellular ligands and receptor ectodomains, \u003cstrong\u003edisulfide bonds\u003c\/strong\u003e and \u003cstrong\u003eglycosylation\u003c\/strong\u003e can influence stability and binding. PTM dependence is target- and assay-specific. Prokaryotic expression typically yields non-glycosylated protein; consider whether eukaryotic PTMs are required for your assay context.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e This protein is produced in a \u003cstrong\u003eprokaryotic (E. coli)\u003c\/strong\u003e system, which typically yields a defined, non-glycosylated form. This can be advantageous for mechanistic studies, binding assays, and antigen\/standard use where mammalian PTMs are not required.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePurification transparency (research credibility):\u003c\/strong\u003e In research-grade recombinant protein production, constructs are commonly purified via affinity and polishing steps (e.g., chromatography) to reduce contaminants and improve batch-to-batch consistency. When present, affinity tags (e.g., His\/GST\/Fc) can simplify purification; tag presence or removal can influence certain binding or structural assays.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eForm and handling context:\u003c\/strong\u003e Lyophilized proteins are frequently used in RUO labs to support stability during storage and shipment, while formulation components and reconstitution conditions can impact solubility and aggregation—important considerations when comparing studies across publications.\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e In cell-based research models, changes in signaling factor levels or responsiveness to \u003cstrong\u003eCxcl15\u003c\/strong\u003e can reflect altered receptor expression, pathway feedback, or microenvironmental cues. Researchers often interpret results alongside orthogonal markers (e.g., pathway phosphorylation, transcriptional programs, or secreted mediator panels) to separate direct ligand effects from downstream network responses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eUsing recombinant protein as a defined reagent:\u003c\/strong\u003e recombinant Cxcl15 is commonly used as a quantitative input for assay calibration, antibody\/ligand binding studies, pathway reconstitution, and controlled perturbation experiments. Researchers often consider isoforms, fragments, or construct boundaries when comparing results across studies.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"50 ug","offer_id":53013972091245,"sku":"P1724-50UG","price":374.4,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53013972124013,"sku":"P1724-200UG","price":748.8,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53013972156781,"sku":"P1724-1MG","price":2246.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/E8_9B_8B_E7_99_BD_b13c042c-7535-4e29-bea2-90a428d1aafb.jpg?v=1770539598","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-cxcl15-bhp10801014","provider":"BioHippo","version":"1.0","type":"link"}