{"product_id":"recombinant-mouse-dihydrolipoyllysine-residue-acetyltransferase-component-of-pyruvate-dehydrogenase-complex-mitochondrial-dlat-bhp10506570","title":"Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eDlat\u003c\/strong\u003e from Mus musculus (Mouse), produced in Mammalian cell (region 86-642aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 86-642aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e Mammalian cell (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex Pyruvate dehydrogenase complex component E2. The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving Dlat\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q8BMF4 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q8BMF4 - NCBI Gene search: Dlat — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Dlat - Ensembl search: Dlat — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Dlat - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053388063085,"sku":"CSB-MP804374MO-1MG","price":3520.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053496263021,"sku":"CSB-MP804374MO-100UG","price":470.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053496295789,"sku":"CSB-MP804374MO-20UG","price":290.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-MP804374MO-SDS.jpg?v=1772177582","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-dihydrolipoyllysine-residue-acetyltransferase-component-of-pyruvate-dehydrogenase-complex-mitochondrial-dlat-bhp10506570","provider":"BioHippo","version":"1.0","type":"link"}