{"product_id":"recombinant-mouse-endoplasmic-reticulum-chaperone-bip-hspa5-bhp10506881","title":"Recombinant Mouse Endoplasmic reticulum chaperone BiP (Hspa5)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eHspa5\u003c\/strong\u003e from Mus musculus (Mouse), produced in E.coli (region 20-655aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 20-655aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as 78 kDa glucose-regulated protein (GRP-78) (Binding-immunoglobulin protein) (BiP) (Heat shock protein 70 family protein 5) (HSP70 family protein 5) (Heat shock protein family A member 5) (Immunoglobulin heavy chain-binding protein) (Grp78). Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10\/ERdj5, probably to facilitate the release of DNAJC10\/ERdj5 from its substrate. Acts as a key repressor of the ERN1\/IRE1-mediated unfolded protein response. In the unstressed endoplasmic reticulum, recruited by DNAJB9\/ERdj4 to the luminal region of ERN1\/IRE1, leading to disrupt the dimerization of ERN1\/IRE1, thereby inactivating ERN1\/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5\/BiP from ERN1\/IRE1, allowing homodimerization and subsequent activation of ERN1\/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum. May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eEndoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10\/ERdj5, probably to facilitate the release of DNAJC10\/ERdj5 from its substrate. Acts as a key repressor of the ERN1\/IRE1-mediated unfolded protein response. In the unstressed endoplasmic reticulum, recruited by DNAJB9\/ERdj4 to the luminal region of ERN1\/IRE1, leading to disrupt the dimerization of ERN1\/IRE1, thereby inactivating ERN1\/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5\/BiP from ERN1\/IRE1, allowing homodimerization and subsequent activation of ERN1\/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum. May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving Hspa5\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P20029 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P20029 - NCBI Gene search: Hspa5 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Hspa5 - Ensembl search: Hspa5 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Hspa5 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053395665261,"sku":"CSB-EP010827MO-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053513204077,"sku":"CSB-EP010827MO-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053513236845,"sku":"CSB-EP010827MO-20UG","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP010827MO-SDS.jpg?v=1772177617","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-endoplasmic-reticulum-chaperone-bip-hspa5-bhp10506881","provider":"BioHippo","version":"1.0","type":"link"}