{"product_id":"recombinant-mouse-galectin-8-bhp11002302","title":"Recombinant Mouse Galectin-8","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Mouse Galectin-8\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eLGALS8 is a prostate-specific antigen that is solely overexpressed in malignant tumors and thus is a supplementary specific identifier of malignancies. LGALS8 is part of the galectin gene family which facilitates both cell-cell and cell matrix interactions in a method parallel to the selectin subgroup of C-type lectins.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Conditions:\u003c\/strong\u003e 1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42g, Sodium citric acid 0.8g, Citric acid 0.055g, D-glucose 2.05g in DW100 ml).\u003cbr\u003e2. Centrifuge at 15000rpm for 10 minutes and wash 4 times with PBS.\u003cbr\u003e3. Dilute packed cells in a 0.5mg\/ml trypsin-EDTA solution to give 4% red cell suspension.\u003cbr\u003e4. Incubate for 1 hour at 37°C and wash 4 times with PBS.\u003cbr\u003e5. Dilute packed cells in PBS to give a 4% red cell suspension.\u003cbr\u003e6. Load 50µl of 0.5%BSA-in-0.15M-NaCl solution and 25µl of 4%-Red-Cell-in-PBS in U shaped wells.\u003cbr\u003e7. Add 25µl of serial diluted galectin protein in PBS to each well plate (Round bottom 96 well plate).\u003cbr\u003e8. Incubate for 30 minutes at room temperature to observe visible agglutination.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"2 ug","offer_id":53038076428653,"sku":"cyt-185-2UG","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10 ug","offer_id":53038335721837,"sku":"cyt-185-10UG","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038335754605,"sku":"cyt-185-1MG","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-lgals8-mouse-CYT-185.png?v=1782158183","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-galectin-8-bhp11002302","provider":"BioHippo","version":"1.0","type":"link"}