{"product_id":"recombinant-mouse-gamma-interferon-inducible-lysosomal-thiol-reductase-ifi30-bhp10514842","title":"Recombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30) is a recombinant protein preparation derived from Mus musculus (Mouse). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 55-230aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 6xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 27 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 90% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eLysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStimulate cultured cells with Ifi30 (dose–response + time-course) and collect lysates.\u003c\/li\u003e\n\u003cli\u003eMeasure downstream markers after Ifi30 treatment (WB\/qPCR\/ELISA readouts).\u003c\/li\u003e\n\u003cli\u003eQuantify Ifi30 using standard curves in plate-based assays (assay development).\u003c\/li\u003e\n\u003cli\u003eRun receptor binding kinetics for Ifi30 by SPR\/BLI (in vitro profiling).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (Q9ESY9) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/Q9ESY9 - NCBI Gene search (Ifi30) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Ifi30 - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Ifi30 - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059270836589,"sku":"CSB-EP872152MO-1MG","price":2062.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059389161837,"sku":"CSB-EP872152MO-100UG","price":480.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059389194605,"sku":"CSB-EP872152MO-20UG","price":256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP872152MO-SDS.jpg?v=1772280180","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-gamma-interferon-inducible-lysosomal-thiol-reductase-ifi30-bhp10514842","provider":"BioHippo","version":"1.0","type":"link"}