{"product_id":"recombinant-mouse-phospholipase-d3-pld3-bhp10507067","title":"Recombinant Mouse Phospholipase D3 (Pld3)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Mus musculus (Mouse): Mouse Phospholipase D3 (Pld3) corresponding to amino acids 1–488. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–488 (488 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 1TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Mus musculus (Mouse). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt O35405. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eMouse Phospholipase D3 (Pld3) (PLD3) is a membrane-associated member of the phospholipase D superfamily, commonly annotated as a type II transmembrane protein with a luminal\/extracellular catalytic region. PLD-family proteins are investigated for roles in lipid metabolism and membrane trafficking, and PLD3 has been discussed in the context of endolysosomal biology. Because catalytic activity and localization can depend on folding and post-translational processing, the expression system and region selection matter for interpreting biochemical readouts. Also reported as: Choline phosphatase 3 (Phosphatidylcholine-hydrolyzing phospholipase D3) (Schwannoma-associated protein 9) (SAM-9) (Sam9) (PLD 3).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEndolysosomal pathway research linking membrane-anchored enzymes to cargo processing and cellular stress responses.\u003c\/li\u003e\n\u003cli\u003eNeurobiology studies exploring associations between PLD-family members, lysosomal function, and neurodegenerative phenotypes (RUO, model systems).\u003c\/li\u003e\n\u003cli\u003eAssay development to disentangle lipase-like activity, nuclease-like activity reports, and localization-dependent functions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntigen generation for antibody production or validation against PLD3 regions.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding or activity-oriented studies that compare constructs\/variants to interpret domain requirements.\u003c\/li\u003e\n\u003cli\u003eLocalization and trafficking studies that relate PLD3 abundance\/distribution to perturbations of endosomal\/lysosomal pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Mouse Phospholipase D3 (Pld3) (O35405) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/O35405\/entry\n- NCBI Gene search: Pld3 Mus musculus — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Pld3%20Mus%20musculus\n- PubMed search: Pld3 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Pld3%20review\n- InterPro search: Pld3 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/Pld3\/\n- Ensembl Gene Summary: Pld3 (Mus_musculus) — Ensembl — https:\/\/www.ensembl.org\/Mus_musculus\/Gene\/Summary?g=Pld3\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207323902317,"sku":"CSB-CF018146MO-100UG","price":2781.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320592064877,"sku":"CSB-CF018146MO-20UG","price":1668.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF018146MO-1.jpg?v=1778623121","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mouse-phospholipase-d3-pld3-bhp10507067","provider":"BioHippo","version":"1.0","type":"link"}