{"product_id":"recombinant-mycolicibacterium-smegmatis-3-oxosteroid-1-dehydrogenase-ksdd-bhp10517201","title":"Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis product is a recombinant strain atcc 700084 \/ mc(2 ksdD protein commonly used as a defined reagent for assay development, interaction studies, and cell-based functional experiments. \u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e 1-569aa — region selection can affect binding epitopes and functional interpretation.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Yeast — expression context can influence folding and post-translational features.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e C-terminal 6xHis-tagged — useful for platform-specific capture or detection workflows.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eCatalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eEnzyme function studies linking activity changes to phenotype across model systems.\u003c\/li\u003e \u003cli\u003eSmall-molecule and cofactor screens using kinetic readouts in purified systems.\u003c\/li\u003e \u003cli\u003eIntegration of enzymatic readouts with omics data to map pathway-level regulation.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eUse as a reagent for consistent dosing across in vitro assays\u003c\/li\u003e \u003cli\u003eGenerate standard curves or spike-in controls in buffer or sample matrix for plate assays\u003c\/li\u003e \u003cli\u003eProfile protein–protein interactions involving ksdD using pull-down or co-binding assays\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eConsider isoforms, processing, or post-translational modifications that may differ from endogenous protein across systems.\u003c\/li\u003e \u003cli\u003eFor tagged or labeled formats, confirm that the tag does not occlude binding sites relevant to your readout.\u003c\/li\u003e \u003cli\u003eUse appropriate negative\/positive controls and orthogonal assays when interpreting binding or activity differences.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt): https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NCBI): https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - NCBI Protein (NIH\/NCBI): https:\/\/www.ncbi.nlm.nih.gov\/protein\/ - Reactome Pathway Database: https:\/\/reactome.org\/ - KEGG Pathway Database: https:\/\/www.kegg.jp\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059862823277,"sku":"CSB-YP368051MVX-1MG","price":2959.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059933438317,"sku":"CSB-YP368051MVX-100UG","price":826.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059933471085,"sku":"CSB-YP368051MVX-20UG","price":436.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-YP368051MVX-SDS.jpg?v=1772294660","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mycolicibacterium-smegmatis-3-oxosteroid-1-dehydrogenase-ksdd-bhp10517201","provider":"BioHippo","version":"1.0","type":"link"}