{"product_id":"recombinant-mycoplasma-pneumoniae-p30-adhesin-p30-bhp10508855","title":"Recombinant Mycoplasma pneumoniae P30 adhesin (p30)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Mycoplasma pneumoniae (strain ATCC 29342 \/ M129): Mycoplasma pneumoniae P30 adhesin (p30) corresponding to amino acids 1–274. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–274 (274 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 2TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Mycoplasma pneumoniae (strain ATCC 29342 \/ M129). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt P75330. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eMycoplasma pneumoniae P30 adhesin (p30) is annotated as an adhesin from Mycoplasma pneumoniae. Adhesins are surface-associated proteins that mediate attachment to host cells or abiotic surfaces, and are central to colonization and host–microbe interactions. Recombinant adhesin regions are commonly used as antigens to study immune recognition, binding partners, and strain variation. Also reported as: 30 kDa adhesin-related protein (Cytadhesin P30).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMapping of adhesion mechanisms and host receptors to understand colonization and tissue tropism.\u003c\/li\u003e\n\u003cli\u003eVaccine antigen discovery and serological research that evaluates immune responses to adhesin domains (RUO).\u003c\/li\u003e\n\u003cli\u003eComparative genomics of adhesin variability and its relationship to pathogenicity and immune evasion.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGeneration of antibodies\/binders to detect surface proteins or probe adhesion-related interactions.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding studies that compare domains\/variants to interpret receptor specificity.\u003c\/li\u003e\n\u003cli\u003eCross-strain comparative studies that contextualize sequence variation and antigenicity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Mycoplasma pneumoniae P30 adhesin (p30) (P75330) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P75330\/entry\n- NCBI Gene search: p30 Mycoplasma pneumoniae — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=p30%20Mycoplasma%20pneumoniae\n- PubMed search: p30 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=p30%20review\n- InterPro search: p30 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/p30\/\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207327572333,"sku":"CSB-CF301931MLW-100UG","price":2781.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320594489709,"sku":"CSB-CF301931MLW-20UG","price":1668.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF301931MLW-SDS.jpg?v=1778623129","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-mycoplasma-pneumoniae-p30-adhesin-p30-bhp10508855","provider":"BioHippo","version":"1.0","type":"link"}