{"product_id":"recombinant-penaeus-monodon-pen-m-1-tm1-tropomyosin-protein-n-his-bhp21409928","title":"Recombinant Penaeus monodon Pen m 1\/TM1\/Tropomyosin Protein, N-His","description":" \u003ch2\u003eBackground\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat is Pen m 1 \/ Tropomyosin?\u003c\/strong\u003e Tropomyosin is a highly conserved \u003cstrong\u003eactin-binding filament protein\u003c\/strong\u003e best known for its role in stabilizing actin filaments and regulating actin–myosin interactions in muscle and muscle-like contractile systems. In crustaceans such as \u003cstrong\u003ePenaeus monodon\u003c\/strong\u003e, the allergen designation \u003cstrong\u003ePen m 1\u003c\/strong\u003e commonly refers to a tropomyosin isoform that is widely used as a reference antigen in seafood-allergen research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eSubcellular localization (research context):\u003c\/strong\u003e Tropomyosin is primarily associated with \u003cstrong\u003eactin filaments\u003c\/strong\u003e in the \u003cstrong\u003ecytoskeleton\u003c\/strong\u003e and, in muscle tissues, within \u003cstrong\u003emyofibrillar thin filaments\u003c\/strong\u003e. Its abundance and filament association make it a robust structural marker in studies of contractile and cytoskeletal organization.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eDomain \/ architecture (why it behaves the way it does):\u003c\/strong\u003e Tropomyosin is a classic \u003cstrong\u003eα-helical coiled-coil\u003c\/strong\u003e protein that typically forms parallel dimers and aligns along the length of actin filaments. This extended coiled-coil architecture contributes to its stability and to the presentation of repeating surface features that can influence antibody recognition and cross-reactivity in comparative studies.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat this recombinant protein represents:\u003c\/strong\u003e This product is a \u003cstrong\u003erecombinant Penaeus monodon Pen m 1 \/ tropomyosin\u003c\/strong\u003e preparation corresponding to \u003cstrong\u003eMet1–Tyr284\u003c\/strong\u003e, expressed in \u003cstrong\u003eE. coli\u003c\/strong\u003e and supplied with an \u003cstrong\u003eN-terminal His tag\u003c\/strong\u003e. As a defined, traceable antigen input, it supports reproducible experimental systems where endogenous material can vary by tissue source, extraction method, and isoform composition.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eCore biological role:\u003c\/strong\u003e Tropomyosin regulates access of myosin to actin and helps specify filament behavior by stabilizing actin and shaping interactions with actin-associated factors. Because this role is foundational to cytoskeletal mechanics, tropomyosin is frequently used in mechanistic studies that connect protein structure to filament function.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhy researchers study Pen m 1:\u003c\/strong\u003e In allergen-focused research settings, Pen m 1 is widely treated as a representative \u003cstrong\u003eshellfish tropomyosin antigen\u003c\/strong\u003e for studying antibody\/IgE binding behavior, antigen standardization, and cross-reactivity logic across related invertebrate tropomyosins. Its strong sequence conservation across arthropods is a major reason it is used for comparative and specificity studies.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eResearch framing:\u003c\/strong\u003e Recombinant Pen m 1 enables controlled experiments where the antigen input is defined and consistent, supporting systematic evaluation of binding specificity, epitope dependence on conformation, and how sequence-level similarity can translate into shared immunoreactivity across species.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eMolecular characteristics relevant to experimental design\u003c\/h2\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein class:\u003c\/strong\u003e Actin-binding cytoskeletal filament protein (tropomyosin family).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStructural behavior:\u003c\/strong\u003e Predominantly \u003cstrong\u003ecoiled-coil α-helical dimer\u003c\/strong\u003e; extended shape and repeating surface features can influence antigen presentation in binding assays.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIsoform considerations:\u003c\/strong\u003e Tropomyosin families often include multiple isoforms; recombinant full-length preparations provide a defined sequence context that can reduce variability compared with heterogeneous endogenous extracts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cstrong\u003ePost-translational considerations:\u003c\/strong\u003e Expression in \u003cstrong\u003eE. coli\u003c\/strong\u003e typically yields a non-glycosylated recombinant form. For tropomyosin antigens, sequence and higher-order coiled-coil conformation are often the primary determinants of recognition in binding experiments, while expression host can still influence folding state and aggregation propensity depending on preparation and formulation.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eExpression and purification context (quality transparency for RUO)\u003c\/h2\u003e\n\n\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e E. coli.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eRegion expressed:\u003c\/strong\u003e Met1–Tyr284 (full-length), with \u003cstrong\u003eN-His\u003c\/strong\u003e tag.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity chromatography; supplied as a lyophilized preparation in a stabilizing formulation to support storage stability and consistent reconstitution for research assays. (Operational storage and shipping details should remain in the dedicated fields, not here.)\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eHow to think about Pen m 1 reagents in research\u003c\/h2\u003e\n\n\u003cp\u003ePen m 1 recombinant protein is best viewed as a \u003cstrong\u003edefined antigen reference material\u003c\/strong\u003e that supports reproducible binding experiments. This is useful when comparing results across platforms, antibody lots, or experimental systems where endogenous antigen sources differ in purity, isoform composition, or extract complexity.\u003c\/p\u003e\n\n\u003cp\u003eBecause tropomyosin is conserved across many invertebrates, observed binding can reflect both target-specific recognition and broader family-level cross-reactivity. Interpreting results is often strengthened by pairing Pen m 1 measurements with sequence-aware controls (e.g., related tropomyosins or designed fragments) when the goal is to distinguish shared versus unique recognition patterns.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 ug","offer_id":53001958228333,"sku":"ZP254012-100UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53001958261101,"sku":"ZP254012-1MG","price":1627.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/recombinant-penaeus-monodon-pen-m-1-tm1-tropomyosin-protein-n-his-bhp21409928","provider":"BioHippo","version":"1.0","type":"link"}