{"product_id":"recombinant-phospho-egfr-antibody-tyr1173-bha17103772","title":"Recombinant phospho-EGFR Antibody (Tyr1173)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e phospho-EGFR antibody supplied as a purified reagent for WB, IHC in Human samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only. The target is commonly annotated with cell membrane, cytoplasm localization context, which may inform staining patterns.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e  Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM269.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, IHC.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A phospho-peptide corresponding to human phospho- EGFR (Tyr1173) was used as the immunogen for this  phospho-EGFR antibody..\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cell membrane, cytoplasm (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e phospho-EGFR is the intended antigen for this primary antibody. Reported biological context includes: This  phospho-EGFR antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173. Subcellular localization information (Cell membrane, cytoplasm) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePost-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.\u003c\/li\u003e\n  \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e\n  \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e\n  \u003cli\u003eImmunohistochemistry (IHC): profile tissue and cell-type distribution in fixed specimens and evaluate spatial heterogeneity in expression.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e\n  \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e\n  \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry (P00533) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P00533\/entry\n- NCBI Gene search ( phospho-EGFR) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=+phospho-EGFR\n- Ensembl search ( phospho-EGFR) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=+phospho-EGFR\n- PubMed search ( phospho-EGFR) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=+phospho-EGFR\n- Reactome pathway search ( phospho-EGFR) — Reactome — https:\/\/reactome.org\/content\/query?q=+phospho-EGFR\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ul","offer_id":53043683131757,"sku":"R20286-0.1ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_09a93c23-90a9-449a-acc6-6ccfb0ba3a4d.jpg?v=1771934414","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-phospho-egfr-antibody-tyr1173-bha17103772","provider":"BioHippo","version":"1.0","type":"link"}