{"product_id":"recombinant-pig-integrin-beta-1-itgb1-partial-bhp10507672","title":"Recombinant Pig Integrin beta-1 (ITGB1), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eITGB1\u003c\/strong\u003e from Sus scrofa (Pig), produced in E.coli (region 140-378aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 140-378aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, Myc (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Fibronectin receptor subunit beta (VLA-4 subunit beta) (CD_antigen: CD29). Integrins alpha-1\/beta-1, alpha-2\/beta-1, alpha-10\/beta-1 and alpha-11\/beta-1 are receptors for collagen. Integrins alpha-1\/beta-1 and alpha-2\/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2\/beta-1, alpha-3\/beta-1, alpha-4\/beta-1, alpha-5\/beta-1, alpha-8\/beta-1, alpha-10\/beta-1, alpha-11\/beta-1 and alpha-V\/beta-1 are receptors for fibronectin. Alpha-4\/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5\/beta-1 is a receptor for fibrinogen. Integrin alpha-1\/beta-1, alpha-2\/beta-1, alpha-6\/beta-1 and alpha-7\/beta-1 are receptors for lamimin. Integrin alpha-6\/beta-1 is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-4\/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9\/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3\/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Integrin alpha-3\/beta-1 provides a docking site for FAP at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3\/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V\/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7\/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis . ITGA4:ITGB1 binds to fractalkine and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site which is distinct from the classical ligand-binding site and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eIntegrins alpha-1\/beta-1, alpha-2\/beta-1, alpha-10\/beta-1 and alpha-11\/beta-1 are receptors for collagen. Integrins alpha-1\/beta-1 and alpha-2\/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2\/beta-1, alpha-3\/beta-1, alpha-4\/beta-1, alpha-5\/beta-1, alpha-8\/beta-1, alpha-10\/beta-1, alpha-11\/beta-1 and alpha-V\/beta-1 are receptors for fibronectin. Alpha-4\/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5\/beta-1 is a receptor for fibrinogen. Integrin alpha-1\/beta-1, alpha-2\/beta-1, alpha-6\/beta-1 and alpha-7\/beta-1 are receptors for lamimin. Integrin alpha-6\/beta-1 is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-4\/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9\/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3\/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Integrin alpha-3\/beta-1 provides a docking site for FAP at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3\/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V\/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7\/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis . ITGA4:ITGB1 binds to fractalkine and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site which is distinct from the classical ligand-binding site and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving ITGB1\u003c\/li\u003e\n\u003cli\u003eBinding interaction studies (e.g., SPR\/BLI or plate-based binding formats)\u003c\/li\u003e\n\u003cli\u003eCell-based stimulation studies with downstream marker readouts (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q9GLP0 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9GLP0 - NCBI Gene search: ITGB1 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ITGB1 - Ensembl search: ITGB1 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ITGB1 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053419782509,"sku":"CSB-EP884398PI-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053562257773,"sku":"CSB-EP884398PI-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053562290541,"sku":"CSB-EP884398PI-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP884398PI-SDS.jpg?v=1772177698","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-pig-integrin-beta-1-itgb1-partial-bhp10507672","provider":"BioHippo","version":"1.0","type":"link"}