{"product_id":"recombinant-plap-antibody-placental-alkaline-phosphatase-n-terminal-bha17103817","title":"Recombinant PLAP Antibody \/ Placental Alkaline Phosphatase \/ N-Terminal","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e PLAP antibody supplied as a purified reagent for WB, IHC-P in Human samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only. The target is commonly annotated with cytoplasmic, cell membrane localization context, which may inform staining patterns.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e  Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM317.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, IHC-P.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A peptide corresponding to the N-terminus of human placental alkaline phosphatase was used as the immunogen for the  PLAP antibody..\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasmic, cell membrane (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e PLAP is the intended antigen for this primary antibody. Reported biological context includes: Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. \u003cbr\u003e\u003cbr\u003ePLAP is a tumor marker, especially in seminoma and ovarian cancer. Subcellular localization information (Cytoplasmic, cell membrane) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eSignal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.\u003c\/li\u003e\n  \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e\n  \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e\n  \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e\n  \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e\n  \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry (P05187) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P05187\/entry\n- NCBI Gene search ( PLAP) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=+PLAP\n- Ensembl search ( PLAP) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=+PLAP\n- PubMed search ( PLAP) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=+PLAP\n- Reactome pathway search ( PLAP) — Reactome — https:\/\/reactome.org\/content\/query?q=+PLAP\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ul","offer_id":53043685032301,"sku":"R20336-0.1ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7931f014-3a82-4baf-a7f9-cb6986a16f27.jpg?v=1771934424","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-plap-antibody-placental-alkaline-phosphatase-n-terminal-bha17103817","provider":"BioHippo","version":"1.0","type":"link"}