{"product_id":"recombinant-plasmodium-berghei-major-facilitator-superfamily-domain-containing-protein-partial-bhp10505909","title":"Recombinant Plasmodium berghei Major facilitator superfamily domain-containing protein, partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Plasmodium berghei: Plasmodium berghei Major facilitator superfamily domain-containing protein, partial (PBK173_000211300) corresponding to amino acids 756–960. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 756–960 (205 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 6TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Plasmodium berghei. Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt A0A0Y9WQZ1. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003ePlasmodium berghei Major facilitator superfamily domain-containing protein, partial (PBK173_000211300) is annotated as a major facilitator superfamily (MFS) domain-containing protein. MFS proteins are ubiquitous secondary transporters that typically use electrochemical gradients to move small molecules across membranes. In parasites and microbes, MFS transporters are often investigated for roles in nutrient uptake, metabolite exchange, and drug susceptibility.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTransporter annotation and substrate prediction using comparative genomics, conserved motif analysis, and structural modeling.\u003c\/li\u003e\n\u003cli\u003eStudies of transporter contributions to antimicrobial\/antiparasitic drug response and resistance mechanisms.\u003c\/li\u003e\n\u003cli\u003eReconstitution and binding-focused approaches to connect specific sequence regions to transport activity (interpretation-focused).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse as a defined antigen for generating reagents that recognize MFS-family proteins or specific loops\/domains.\u003c\/li\u003e\n\u003cli\u003eBiochemical or biophysical characterization of an expressed region to support structure–function hypotheses.\u003c\/li\u003e\n\u003cli\u003eComparative expression studies across life-cycle stages or conditions to contextualize transporter regulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Plasmodium berghei Major facilitator superfamily domain-containing protein, partial (PBK173_000211300) (A0A0Y9WQZ1) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/A0A0Y9WQZ1\/entry\n- NCBI Gene search: PBK173_000211300 Plasmodium berghei — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PBK173_000211300%20Plasmodium%20berghei\n- PubMed search: PBK173_000211300 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PBK173_000211300%20review\n- InterPro search: PBK173_000211300 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/PBK173_000211300\/\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207322722669,"sku":"CSB-CF2221PLH-100UG","price":1462.6,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320588460397,"sku":"CSB-CF2221PLH-20UG","price":904.34,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF2221PLH-SDS.jpg?v=1778623121","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-plasmodium-berghei-major-facilitator-superfamily-domain-containing-protein-partial-bhp10505909","provider":"BioHippo","version":"1.0","type":"link"}