{"product_id":"recombinant-priestia-megaterium-bifunctional-cytochrome-p450-nadph-p450-reductase-cyp102a1-partial-bhp10509610","title":"Recombinant Priestia megaterium Bifunctional cytochrome P450\/NADPH--P450 reductase (cyp102A1), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant Priestia megaterium Bifunctional cytochrome P450\/NADPH--P450 reductase (cyp102A1), partial is a recombinant protein reagent derived from Priestia megaterium (strain ATCC 14581 \/ DSM 32 \/ CCUG 1817 \/ JCM 2506 \/ NBRC 15308 \/ NCIMB 9376 \/ NCTC 10342 \/ NRRL B-14308 \/ VKM B-512 \/ Ford 19) (Bacillus megaterium) and produced in E.coli. It is commonly used to support Cancer research by enabling binding assays, assay development and protein–protein interaction studies in controlled in vitro settings.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 2-472aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli. Expression host can influence folding and the presence\/absence of post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eTag \/ fusion:\u003c\/strong\u003e N-terminal 10xHis-tagged and C-terminal Myc-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight (reported):\u003c\/strong\u003e 61.2 kDa. Apparent size may vary with tags, processing, and gel conditions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWhen comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThe gene commonly associated with this target is \u003cstrong\u003ecyp102A1\u003c\/strong\u003e. cyp102A1 refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Priestia megaterium (strain ATCC 14581 \/ DSM 32 \/ CCUG 1817 \/ JCM 2506 \/ NBRC 15308 \/ NCIMB 9376 \/ NCTC 10342 \/ NRRL B-14308 \/ VKM B-512 \/ Ford 19) (Bacillus megaterium) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt\/NCBI) and the recent literature for the specific organism and isoform.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eMapping pathway dependencies and signaling networks that drive tumor growth and drug resistance.\u003c\/li\u003e \u003cli\u003eDeveloping and benchmarking biomarker assays (e.g., immunoassays or binding reagents) for candidate targets.\u003c\/li\u003e \u003cli\u003eCharacterizing protein variants, domains, or interaction partners relevant to targeted therapeutics and precision oncology.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eRelevance:\u003c\/strong\u003e Functions as a fatty acid monooxygenase . Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions . Shows activity toward medium and long-chain fatty acids, with optimum chain lengths of 12, 14 and 16 carbons (lauric, myristic, and palmitic acids). Able to metabolize some of these primary metabolites to secondary and tertiary products . Marginal activity towards short chain lengths of 8-10 carbons . Hydroxylates highly branched fatty acids, which play an essential role in membrane fluidity regulation . Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain . Involved in inactivation of quorum sensing signals of other competing bacteria by oxidazing efficiently acyl homoserine lactones (AHLs), molecules involved in quorum sensing signaling pathways, and their lactonolysis products acyl homoserines (AHs) .\u003c\/p\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAssay and standard development for immunoassays or binding-based detection methods.\u003c\/li\u003e \u003cli\u003eProtein–protein interaction studies (e.g., receptor–ligand or complex assembly) using purified components.\u003c\/li\u003e \u003cli\u003eStructure–function analysis, including domain mapping or evaluation of sequence variants.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative\/positive controls and, where possible, orthogonal assay formats that support the same conclusion.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eRecombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.\u003c\/li\u003e \u003cli\u003eTag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.\u003c\/li\u003e \u003cli\u003eSpecies and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.\u003c\/li\u003e \u003cli\u003eE. coli expression can limit eukaryotic post-translational modifications; for modification-dependent biology, interpret results accordingly.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry for P14779 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P14779\/entry - NCBI Gene search (cyp102A1) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=cyp102A1 - PubMed search (cyp102A1) — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=cyp102A1 - RCSB PDB search (cyp102A1) — RCSB PDB — https:\/\/www.rcsb.org\/search?query=cyp102A1 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53065293267309,"sku":"CSB-EP320426FSZ-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53065440264557,"sku":"CSB-EP320426FSZ-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53065440297325,"sku":"CSB-EP320426FSZ-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP320426FSZ-SDS.jpg?v=1772476470","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-priestia-megaterium-bifunctional-cytochrome-p450-nadph-p450-reductase-cyp102a1-partial-bhp10509610","provider":"BioHippo","version":"1.0","type":"link"}