{"product_id":"recombinant-rat-interleukin-13-rrtil-13-bhp11300398","title":"Recombinant Rat Interleukin 13 (rRtIL-13)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein\u003c\/span\u003e\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eInterleukin-13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 shares functional properties with IL-4 and is a key mediator in allergic inflammation and fibrosis models.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression System: E. coli\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant rat IL-13 is expressed in Escherichia coli as a single non-glycosylated polypeptide chain containing 110 amino acids with an approximate molecular weight of 12.1 kDa. The protein is purified to \u0026gt;95% as determined by SDS-PAGE and HPLC analyses and is supplied as a sterile-filtered, white lyophilized powder.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003ctd\u003eEscherichia coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 12.1 kDa, a single non-glycosylated polypeptide chain containing 110 amino acids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmino Acid Sequence\u003c\/th\u003e\n\u003ctd style=\"word-break:break-all\"\u003ePVRRSTSPPV ALRELIEELS NITQDQKTSL CNSSMVWSVD LTAGGFCAAL ESLTNISSCN AIHRTQRILN GLCNQKASDV ASSPPDTKIE VAQFISKLLN YSKQLFRYGH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level\u003c\/th\u003e\n\u003ctd\u003eLess than 1EU\/μg of rRtIL-13 as determined by LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePhysical Appearance\u003c\/th\u003e\n\u003ctd\u003eSterile Filtered White lyophilized (freeze-dried) powder.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eThis lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eFunctional Activity\u003c\/h2\u003e\n\u003cp\u003eMeasured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 ng\/mL.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eThis product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore additional recombinant cytokines and growth factors from Bioworld Technology Inc, or contact us for matched antibodies and ELISA kits for IL-13 research.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"2 ug","offer_id":53719338975597,"sku":"PR3009-2UG","price":163.0,"currency_code":"USD","in_stock":true},{"title":"10 ug","offer_id":53719339172205,"sku":"PR3009-10UG","price":307.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53719339204973,"sku":"PR3009-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nopic_bb33dc5d-e459-4650-89c2-72766aecdc30.jpg?v=1786566010","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-rat-interleukin-13-rrtil-13-bhp11300398","provider":"BioHippo","version":"1.0","type":"link"}