{"product_id":"recombinant-saccharomyces-cerevisiae-nucleoside-diphosphate-kinase-ynk1-bhp10507857","title":"Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase (YNK1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eYNK1\u003c\/strong\u003e from Saccharomyces cerevisiae (strain ATCC 204508 \/ S288c) (Baker's yeast), produced in Yeast (region 1-153aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-153aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e Yeast (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as NDK (NDP kinase) (NDK1) (YNK). Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Required for repair of UV radiation- and etoposide-induced DNA damage.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eMajor role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Required for repair of UV radiation- and etoposide-induced DNA damage\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving YNK1\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P36010 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P36010 - NCBI Gene search: YNK1 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=YNK1 - Ensembl search: YNK1 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=YNK1 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053425811821,"sku":"CSB-YP015889SVG-1MG","price":2959.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053572940141,"sku":"CSB-YP015889SVG-100UG","price":826.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053572972909,"sku":"CSB-YP015889SVG-20UG","price":436.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-YP015889SVG-SDS.jpg?v=1772177715","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-saccharomyces-cerevisiae-nucleoside-diphosphate-kinase-ynk1-bhp10507857","provider":"BioHippo","version":"1.0","type":"link"}