{"product_id":"recombinant-saccharomyces-cerevisiae-site-specific-recombinase-flp-flp1-bhp10505239","title":"Recombinant SaccharoMyces cerevisiae Site-specific recombinase Flp (FLP1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant SaccharoMyces cerevisiae Site-specific recombinase Flp (FLP1) is a recombinant protein reagent for research-use applications such as assay development, binding studies, and mechanistic experiments. It corresponds to \u003cstrong\u003eFLP1\u003c\/strong\u003e (SaccharoMyces cerevisiae (strain ATCC 204508 \/ S288c) (Baker's yeast)) and is intended for RUO workflows where a defined protein standard or functional input is needed.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (expression context can influence folding and PTMs).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e 1-423aa (region choice can affect activity and binding readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate(s)\/tag:\u003c\/strong\u003e N-terminal 6xHis-tagged (can support detection or purification depending on format).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 54.1 kDa (useful for interpreting gel migration and size-exclusion profiles).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eWhen comparing results across assays, consider that expression system and expressed region can alter glycosylation, disulfide formation, and oligomerization state, which may shift apparent potency or binding behavior in vitro.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003ePart of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is \u0026gt;144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReagent standardization: using recombinant proteins as reference materials for quantitative calibration and cross-study comparability.\u003c\/li\u003e\n\u003cli\u003eInteraction-focused studies: mapping binding partners, affinity changes, and structure–function relationships across variants or domains.\u003c\/li\u003e\n\u003cli\u003eMulti-omic readouts: combining recombinant perturbations with transcript, protein, and functional endpoints to connect mechanism to phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssay development and validation: use as a defined input or standard where protein identity is required.\u003c\/li\u003e\n\u003cli\u003eBinding studies: evaluate interaction strength and specificity using plate-based or biophysical formats.\u003c\/li\u003e\n\u003cli\u003eCell-response profiling: add protein to cultured cells and interpret downstream marker changes with appropriate controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpretation is most robust when signal changes are evaluated relative to matched controls (buffer-only, unrelated protein controls, or pathway controls) and when readouts are compared across dose and time.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eIsoforms and PTMs can influence binding and activity; ensure the expressed region and expression system match your experimental needs.\u003c\/li\u003e\n\u003cli\u003eSpecies differences may affect receptor binding or antibody recognition; confirm species\/source alignment with your model.\u003c\/li\u003e\n\u003cli\u003eUse concept-level controls such as negative controls (no protein), matrix controls, or orthogonal readouts to support conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt keyword search: https:\/\/www.uniprot.org\/uniprotkb?query=FLP1 - NCBI Gene search: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FLP1 - PubMed search: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FLP1 - Ensembl search: https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FLP1 - Reactome Pathway Browser: https:\/\/reactome.org\/content\/query?q=FLP1 --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053082304877,"sku":"CSB-EP361171SVG-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053263577453,"sku":"CSB-EP361171SVG-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053263610221,"sku":"CSB-EP361171SVG-20UG","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP361171SVG-SDS.jpg?v=1772173027","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-saccharomyces-cerevisiae-site-specific-recombinase-flp-flp1-bhp10505239","provider":"BioHippo","version":"1.0","type":"link"}