{"product_id":"recombinant-salmo-salar-vertebrate-ancient-opsin-partial-bhp10505911","title":"Recombinant Salmo salar Vertebrate ancient opsin, partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Salmo salar (Atlantic salmon): Salmo salar Vertebrate ancient opsin, partial (Recombinant Salmo salar Vertebrate ancient opsin protein, partial) corresponding to amino acids 1–75. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–75 (75 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 1TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Salmo salar (Atlantic salmon). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt O13018. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eSalmo salar Vertebrate ancient opsin, partial (Recombinant Salmo salar Vertebrate ancient opsin protein, partial) is an opsin-family protein, part of the broader rhodopsin-like GPCR superfamily that binds a chromophore and mediates light-dependent signaling in visual and non-visual photoreception. Vertebrate ancient opsins have been reported in multiple vertebrates and are studied for roles in non-image-forming light sensing and physiological regulation. Opsins are multi-pass membrane proteins; fragments may be used for epitope-focused studies, while full-length, correctly folded receptor–chromophore complexes are typically required for photochemical functional work. Also reported as: Vertebrate ancient opsin.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMapping non-visual photoreception pathways and their links to circadian, endocrine, and behavioral regulation.\u003c\/li\u003e\n\u003cli\u003eComparative evolution of opsin subfamilies across vertebrates and functional divergence of spectral tuning.\u003c\/li\u003e\n\u003cli\u003eStructural and mutational analyses connecting key residues to chromophore binding and signaling state transitions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntibody development and immunodetection studies targeting defined opsin regions.\u003c\/li\u003e\n\u003cli\u003eComparative studies of opsin sequence features across species to support evolutionary or functional hypotheses.\u003c\/li\u003e\n\u003cli\u003eInteraction studies with signaling partners where region selection helps interpret specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Salmo salar Vertebrate ancient opsin, partial (Recombinant Salmo salar Vertebrate ancient opsin protein, partial) (O13018) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/O13018\/entry\n- NCBI Gene search: Recombinant Salmo salar Vertebrate ancient opsin protein, partial Salmo salar — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Recombinant%20Salmo%20salar%20Vertebrate%20ancient%20opsin%20protein%2C%20partial%20Salmo%20salar\n- PubMed search: Recombinant Salmo salar Vertebrate ancient opsin protein, partial review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Recombinant%20Salmo%20salar%20Vertebrate%20ancient%20opsin%20protein%2C%20partial%20review\n- InterPro search: Recombinant Salmo salar Vertebrate ancient opsin protein, partial — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/Recombinant%20Salmo%20salar%20Vertebrate%20ancient%20opsin%20protein%2C%20partial\/\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207325507949,"sku":"CSB-CF517517SWI-100UG","price":1462.6,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320590262637,"sku":"CSB-CF517517SWI-20UG","price":904.34,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF517517SWI-SDS.jpg?v=1778623121","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-salmo-salar-vertebrate-ancient-opsin-partial-bhp10505911","provider":"BioHippo","version":"1.0","type":"link"}