{"product_id":"recombinant-thermus-aquaticus-dna-mismatch-repair-protein-muts-bhp11005604","title":"Recombinant Thermus Aquaticus DNA Mismatch Repair Protein MutS","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Thermus Aquaticus DNA Mismatch Repair Protein MutS\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eThe MutS DNA Mismatch Protein Identifies heteroduplex DNAs comprising mis-paired or unpaired bases. The MutS DNA Mismatch Protein binds in vitro to heteroduplex DNAs comprising mis-paired or unpaired bases over a varied temperature range from 4-70 °C and obtains a thermostable ATPase activity. The MutS DNA Mismatch Protein is active at temperature between 0 to 75°C. Since The MutS DNA Mismatch Protein competently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is suitable for sensing these mutations. Mutations can be distinguished in polyacrylamide gels or on a solid phase such as Ni agarose or beads or magnetic Ni-NTA particles.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReaction Conditions:\u003c\/strong\u003e 1. After first round PCR, purify PCR fragments using Qiagen QIAquick PCR purification kit with elution in dH 2 O.\u003cbr\u003e2. Dilute PCR product to 250 ng\/µl in 10 mM Tris–HCl, pH 7.8, 50 mM NaCl and heat to 95 °C for 5 min followed by cooling at 0.1°C \/s to 25 °C.\u003cbr\u003e3. Add reaction buffer (100mM KCl, 50 mM Tris-HCl, pH 8.5, 1 0 mM MgCl 2 , 0.1 mM EDTA, 1 mM DTT, 2% Glycerol) to annealed PCR product, adjust DNA concentration to 11.5 ng\/µl, add 6 × His-MutS dimers to 950 nM.\u003cbr\u003e4. Incubate the mixture at room temperature for 10 min, then add an equal volume of Ni-NTA beads pre-equilibrated with 1×binding buffer, and incubate for 30 min at room temperature.\u003cbr\u003e5. Gently spin down beads and save supernatant for subsequent processing (second round PCR, cloning etc).\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"10 ug","offer_id":53038169751917,"sku":"pro-2573-10UG","price":60.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53038518927725,"sku":"pro-2573-50UG","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038518960493,"sku":"pro-2573-1MG","price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-muts-dna-mismatch-repair-protein-PRO-2573.png?v=1782158369","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-thermus-aquaticus-dna-mismatch-repair-protein-muts-bhp11005604","provider":"BioHippo","version":"1.0","type":"link"}