{"product_id":"recombinant-thermus-aquaticus-dna-polymerase-i-thermostable-pola-i614n-l616i-partial-bhp10508357","title":"Recombinant Thermus aquaticus DNA polymerase I, thermostable (polA) (I614N,L616I), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003epolA\u003c\/strong\u003e from Thermus aquaticus, produced in E.coli (region 293-832aa(I614,L616I)). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 293-832aa(I614,L616I) (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Taq polymerase 1. This target is studied in diverse cellular pathways; consult the linked sources for gene\/protein context.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving polA\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P19821 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P19821 - NCBI Gene search: polA — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=polA - Ensembl search: polA — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=polA - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053441671533,"sku":"CSB-EP323156TNN1(M1)-1MG","price":2632.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053604168045,"sku":"CSB-EP323156TNN1(M1)-100UG","price":895.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053604200813,"sku":"CSB-EP323156TNN1(M1)-20UG","price":554.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP323156TNN1_M1_-SDS.jpg?v=1772177775","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-thermus-aquaticus-dna-polymerase-i-thermostable-pola-i614n-l616i-partial-bhp10508357","provider":"BioHippo","version":"1.0","type":"link"}