{"product_id":"recombinant-thermus-phage-tsp4-peptidoglycan-hydrolase-protein-c-his-bhp21402830","title":"Recombinant Thermus phage TSP4 Peptidoglycan hydrolase Protein, C-His","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eTarget identity:\u003c\/strong\u003e\u003cstrong\u003eTHERMUS\u003c\/strong\u003e is a enzyme. It is typically cytosolic, nuclear, or organelle-localized (depends on isoform).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTHERMUS\u003c\/strong\u003e is provided as a recombinant protein reagent for \u003cstrong\u003eresearch use only\u003c\/strong\u003e. Recombinant proteins are commonly used as defined molecular inputs in biochemical and cell-free systems, enabling controlled interrogation of binding, activity, and pathway-relevant interactions.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProtein identity context:\u003c\/strong\u003e THERMUS (expression region Met1-Lys166; approx. molecular weight 19.57 kDa).\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eTHERMUS\u003c\/strong\u003e supports biochemical transformations that can be read out as changes in substrate\/product balance or signaling intermediates. Recombinant enzymes enable controlled reconstitution experiments and mechanistic interrogation with defined inputs. This target is frequently explored in \u003cstrong\u003eMetabolism \u0026amp; Enzymology\u003c\/strong\u003e research contexts.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003eKey molecular attributes can influence binding behavior, stability, and assay background—especially for multimeric, disulfide-rich, or PTM-dependent targets.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Bacillus Subtilis\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e Met1-Lys166\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 19.57 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePost-translational considerations:\u003c\/strong\u003e Expression host can influence folding and PTMs (e.g., glycosylation, disulfide bonds), which may impact stability or binding depending on protein class. For enzymes, cofactor binding state and oligomerization can also shape apparent activity in reconstituted assays.\u003c\/p\u003e\u003ch2\u003eStructural and biochemical features\u003c\/h2\u003e\u003cp\u003eEnzyme behavior can be influenced by oligomerization, cofactor state, and local microenvironment, which may change apparent kinetics in simplified assay formats.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e Bacillus Subtilis. Expression host selection can influence folding and PTM state, which may affect activity or binding in different assay formats.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity-chromatography. Purification approach and formulation influence sample homogeneity and background signal in downstream biochemical measurements.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eEndotoxin consideration:\u003c\/strong\u003e Reported endotoxin level is Please contact with the lab for this information.; this parameter can matter when recombinant proteins are used in cell-based systems sensitive to innate immune activation.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details..\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e Enzymatic readouts can reflect both abundance and catalytic state. Orthogonal measurements (substrate levels, product formation, and pathway markers) help clarify whether changes arise from expression, inhibition, or cofactor availability.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 ug","offer_id":53000901689709,"sku":"VK423012-100UG","price":361.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53000901722477,"sku":"VK423012-1MG","price":2032.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/recombinant-thermus-phage-tsp4-peptidoglycan-hydrolase-protein-c-his-bhp21402830","provider":"BioHippo","version":"1.0","type":"link"}