{"product_id":"recombinant-tilapia-leptin-a-bhp11005880","title":"Recombinant Tilapia Leptin-A","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Tilapia Leptin-A\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eLeptin is a protein hormone. It is mainly produced in adipose cells that regulate energy homeostasis by restraining hunger. Leptin ties to nuclear receptors in the hypothalamus (arcuate nucleus). Similar to insulin resistance in type II diabetes, in obesity there is a decrease in the sensitivity towards leptin, ending in a failure to identify satiety, even in high levels of energy stores or leptin itself. Full-length cDNA encoding 2 leptin sequences (tLepA and tLepB) and 1 leptin receptor sequence (tLepR) exists in tilapia (Oreochromis niloticus). The full-length cDNA of tLepR is 3423 bp, encoding a protein of 1140 amino acid which contained all functionally important domains conserved among vertebrate leptin receptors. The cDNAs of tLepA and tLepB are 486 bp and 459 bp in length, encoding proteins of 161 aa and 152 aa, respectively. The three-dimensional structures of tLepA and tLepB demonstrates strong conservation of tertiary structure with that of human leptin comprised of 4 helixes.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eQuality notes\u003c\/h3\u003e\n\u003cp\u003eProtein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.33 for 1 mg\/ml Leptin-A Tilapia as the extinction coefficient for a 0.1% (1mg\/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.\u003c\/p\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"20 ug","offer_id":53038176371053,"sku":"cyt-1109-20UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53038532395373,"sku":"cyt-1109-100UG","price":260.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038532428141,"sku":"cyt-1109-1MG","price":2400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-leptin-a-CYT-1109.png?v=1782158382","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-tilapia-leptin-a-bhp11005880","provider":"BioHippo","version":"1.0","type":"link"}