{"product_id":"recombinant-tityus-serrulatus-alpha-mammal-toxin-ts2-bhp10506997","title":"Recombinant Tityus serrulatus Alpha-mammal toxin Ts2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eTs2 (Alpha)\u003c\/strong\u003e from Tityus serrulatus (Brazilian scorpion), produced in Baculovirus (region 1-62aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-62aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e Baculovirus (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, Myc (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as P-Mice-beta* NaTx5.1 (Tityustoxin II) (Toxin T1-IV) (TsTX III-8) (TsTX-III). Alpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin acts on Nav1.2\/SCN2A, Nav1.3\/SCN3A, Nav1.5\/SCN5A, Nav1.6\/SCN8A and Nav1.7\/SCN9A voltage-gated sodium channels, with the highest affinity for Nav1.3\/SCN3A, followed by Nav1.6\/SCN8A and Nav1.7\/SCN9A which are affected almost equally. Interestingly, shows a significant shift of the voltage dependence of activation for Nav1.3\/SCN3A that is characteristic of beta-toxins . In addition, in presence of LPS, this toxin inhibits the release of NO, IL-6 and TNF-alpha in J774.1 cells . Further, in the absence of LPS, it stimulates the production of the anti-inflammatory cytokine IL-10 . This toxin is active on mammals.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAlpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin acts on Nav1.2\/SCN2A, Nav1.3\/SCN3A, Nav1.5\/SCN5A, Nav1.6\/SCN8A and Nav1.7\/SCN9A voltage-gated sodium channels, with the highest affinity for Nav1.3\/SCN3A, followed by Nav1.6\/SCN8A and Nav1.7\/SCN9A which are affected almost equally. Interestingly, shows a significant shift of the voltage dependence of activation for Nav1.3\/SCN3A that is characteristic of beta-toxins . In addition, in presence of LPS, this toxin inhibits the release of NO, IL-6 and TNF-alpha in J774.1 cells . Further, in the absence of LPS, it stimulates the production of the anti-inflammatory cytokine IL-10 . This toxin is active on mammals.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving Ts2 (Alpha)\u003c\/li\u003e\n\u003cli\u003eBinding interaction studies (e.g., SPR\/BLI or plate-based binding formats)\u003c\/li\u003e\n\u003cli\u003eCell-based stimulation studies with downstream marker readouts (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P68410 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P68410 - NCBI Gene search: Ts2 (Alpha) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Ts2%20(Alpha) - Ensembl search: Ts2 (Alpha) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Ts2%20(Alpha) - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053399466349,"sku":"CSB-BP300103TON-1MG","price":3278.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053520347501,"sku":"CSB-BP300103TON-100UG","price":1478.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053520380269,"sku":"CSB-BP300103TON-20UG","price":528.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-BP300103TON-SDS.jpg?v=1772177626","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-tityus-serrulatus-alpha-mammal-toxin-ts2-bhp10506997","provider":"BioHippo","version":"1.0","type":"link"}