{"product_id":"recombinant-tobacco-etch-virus-protease-bhp11001646","title":"Recombinant Tobacco Etch Virus Protease","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Tobacco Etch Virus Protease\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eTEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G\/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCleavage Conditions:\u003c\/strong\u003e A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and\/or more rTEV.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"100 IU","offer_id":53038067876205,"sku":"pro-585-100IU","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500 IU","offer_id":53038318616941,"sku":"pro-585-500IU","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1000 IU","offer_id":53038318649709,"sku":"pro-585-1000IU","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-tev-protease-PRO-585.png?v=1782158164","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-tobacco-etch-virus-protease-bhp11001646","provider":"BioHippo","version":"1.0","type":"link"}