{"product_id":"recombinant-yersinia-enterocolitica-protein-yopb-yopb-bhp10503911","title":"Recombinant Yersinia enterocolitica Protein YopB (yopB)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Yersinia enterocolitica: Yersinia enterocolitica Protein YopB (yopB) corresponding to amino acids 1–401. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–401 (401 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 2TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Yersinia enterocolitica. Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt P37131. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eYersinia enterocolitica Protein YopB (yopB) is derived from Yersinia enterocolitica and is associated with bacterial virulence. YopB-family proteins are components of the type III secretion system (T3SS) translocon in several Yersinia species, where they help form pores that deliver effector proteins into host cells. Functional notes for this entry state it plays a role in virulence. Virulence factors are frequently studied for their interactions with host membranes and immune responses, and defined recombinant regions can support mechanistic and immunological studies. Also reported as: yopB; Protein YopB.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHost–pathogen interaction research mapping T3SS assembly and translocon pore formation at host membranes.\u003c\/li\u003e\n\u003cli\u003eIdentification of neutralizing antibodies or inhibitors that disrupt translocation (RUO).\u003c\/li\u003e\n\u003cli\u003eComparative analyses of virulence factor variation across strains and its impact on host range and immune evasion.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse as a defined antigen for antibody generation or serology-oriented research assays (RUO).\u003c\/li\u003e\n\u003cli\u003eBiochemical interaction studies with host membrane components or partner T3SS proteins.\u003c\/li\u003e\n\u003cli\u003eComparative studies of YopB-like proteins across Yersinia and related pathogens.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Yersinia enterocolitica Protein YopB (yopB) (P37131) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P37131\/entry\n- NCBI Gene search: yopB Yersinia enterocolitica — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=yopB%20Yersinia%20enterocolitica\n- PubMed search: yopB review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=yopB%20review\n- InterPro search: yopB — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/yopB\/\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207322231149,"sku":"CSB-CF334228YAQ-100UG","price":2781.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320588329325,"sku":"CSB-CF334228YAQ-20UG","price":1668.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF334228YAQ-SDS.jpg?v=1778623120","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-yersinia-enterocolitica-protein-yopb-yopb-bhp10503911","provider":"BioHippo","version":"1.0","type":"link"}