{"product_id":"renin-receptor-antibody-atp6ap2-bha17136253","title":"Renin receptor Antibody \/ ATP6AP2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRenin receptor Antibody \/ ATP6AP2 is a anti-ATP6AP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER, Lysosome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP6AP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRenin receptor antibody detects the (Pro)renin receptor, also known as ATP6AP2, a multifunctional membrane protein encoded by the ATP6AP2 gene on chromosome Xp11.4. ATP6AP2 serves dual roles as a receptor for renin and prorenin and as an accessory subunit of the vacuolar H+-ATPase (V-ATPase) complex. It is a type I transmembrane protein expressed in kidney, heart, brain, and vascular tissues, where it regulates blood pressure, renal physiology, and cellular acidification. ATP6AP2 plays crucial roles in the renin-angiotensin system (RAS) and lysosomal proton pump function.\u003cbr\u003e\u003cbr\u003eFunctionally, ATP6AP2 binds both renin and its precursor prorenin, enhancing angiotensin I generation and activating intracellular signaling pathways independent of angiotensin II. This binding promotes cell proliferation, fibrosis, and inflammation through ERK1\/2 and MAPK activation. In the kidney, ATP6AP2 is localized to juxtaglomerular and distal tubular cells, where it regulates renin signaling and fluid balance. As a V-ATPase accessory subunit, it stabilizes proton pump assembly, contributing to lysosomal acidification and endosomal trafficking.\u003cbr\u003e\u003cbr\u003eStructurally, ATP6AP2 contains an extracellular renin-binding domain, a single transmembrane segment, and a short cytoplasmic tail that mediates interactions with the V-ATPase complex. It is classified within the V-ATPase assembly factor family, sharing homology with ATP6AP1. Co-localization studies show ATP6AP2 at the plasma membrane, endosomes, and lysosomes, reflecting its multifunctional localization and dual biological roles. Known interaction partners include renin, prorenin, and the V-ATPase V0 domain subunits.\u003cbr\u003e\u003cbr\u003eATP6AP2 is widely expressed in cardiovascular and neural tissues, with developmental expression detected in embryonic heart and brain, suggesting roles in organogenesis. In the central nervous system, ATP6AP2 modulates neuronal development, synaptic transmission, and autophagy regulation. Dysregulation of ATP6AP2 expression or mutation causes X-linked intellectual disability and Parkinsonism with spasticity (ATP6AP2-related disorder). In vascular smooth muscle and cardiac fibroblasts, ATP6AP2 signaling contributes to hypertension, cardiac hypertrophy, and fibrosis through MAPK and TGF-beta activation.\u003cbr\u003e\u003cbr\u003eClinically, elevated ATP6AP2 expression is associated with cardiovascular diseases, diabetic nephropathy, and tumor progression. It influences Wnt and Notch signaling pathways that regulate proliferation and differentiation. Pathway associations include renin-angiotensin signaling, proton transport via V-ATPase, and lysosomal degradation. Tissue-specific studies show high ATP6AP2 activity in renal epithelia and cardiomyocytes, coordinating hormonal and metabolic regulation.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using Renin receptor antibody reveals membrane and cytoplasmic localization in kidney and cardiac tissues. The Renin receptor antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP6AP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP6AP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP6AP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP6AP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP6AP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323558253,"sku":"FY13351","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_92b8d9ff-edb6-4c8d-b476-8b771fb32a62.jpg?v=1782237102","url":"https:\/\/www.ebiohippo.com\/products\/renin-receptor-antibody-atp6ap2-bha17136253","provider":"BioHippo","version":"1.0","type":"link"}