{"product_id":"reporter-negative-control-lentivirus-bhv19400066","title":"Reporter Negative Control Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNegative controls are essential for the correct interpretation of transcription factor reporter experiments. Reporter assays measure pathway-specific transcription by placing a fluorescent or luminescent gene under the control of defined response elements, but reporter cells can also show basal signal that is independent of the pathway under study. A matched negative control, identical to the active reporter except that it lacks the pathway-specific response elements, defines the background level of reporter expression. Comparing the active reporter against this control confirms whether a measured signal reflects genuine pathway activity rather than nonspecific or off-target effects. This is particularly important for reporters with strong basal activity or for weakly inducible pathways, where careful background correction underpins reliable quantitative analysis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Reporter Negative Control Lentivirus is designed for use alongside transcription factor reporter lentiviruses to define background reporter activity. It contains all components of a standard TF reporter construct except the response elements that otherwise drive pathway-specific reporter expression, providing a baseline measurement against which true pathway signal is compared. The construct carries a reporter gene (d2GFP, EGFP, GFP, mCherry, RFP, or firefly luciferase) and an EF1a-driven selection marker (Blasticidin or Puromycin) for straightforward establishment of stable cell lines. It helps confirm that strong basal reporter activity is response-element dependent and that weak induction is pathway specific rather than off-target. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it transduces primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625419117,"sku":"LTV-0071-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310897488237,"sku":"LTV-0071-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310897521005,"sku":"LTV-0071-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897553773,"sku":"LTV-0071-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310897586541,"sku":"LTV-0071-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310897619309,"sku":"LTV-0071-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-general-2_bf5ef25b-dc7c-4937-9197-778ea18221c5.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/reporter-negative-control-lentivirus-bhv19400066","provider":"BioHippo","version":"1.0","type":"link"}